cAMP-elevating agents such as forskolin and vasoactive intestinal peptide induce liquid secretion by tracheobronchial submucosal glands. This pathway is thought to be CFTR dependent and thus defective in cystic fibrosis; however, the ionic mechanism that drives this secretion process is incompletely understood. To better define this mechanism, we studied the effects of ion transport inhibitors on the forskolin-induced liquid secretion response (Jv) of porcine distal bronchi. The forskolin-induced Jv was driven by a combination of bumetanide-sensitive Cl– secretion and DIDS-sensitive HCO3– secretion. When Cl– secretion was inhibited with bumetanide, Na+/H+ exchange-dependent HCO3– secretion was apparently induced to compensate for the loss of Cl– secretion. The forskolin-induced Jv was significantly inhibited by the anion channel blockers 5-nitro-2-(3-phenylpropylamino)benzoic acid, diphenylamine-2-carboxylate, and glibenclamide. We conclude that the forskolin-induced Jv shares many characteristics of cholinergically induced secretion except for the presence of a DIDS-sensitive component. Although the identity of the DIDS-sensitive component is unclear, we speculate that it represents a basolateral membrane Na+-HCO3– cotransporter or an Na+-dependent anion exchanger, which could account for transepithelial HCO3– secretion.
|Journal||American Journal of Physiology: Lung Cellular and Molecular Physiology|
|Publication status||Published - Jan 2006|
- Cystic fibrosis
- Airway liquid secretion
- Submucosal glands
Ballard, S. T., Trout, L., Garrison, J., & Inglis, S. (2006). Ionic mechanism of forskolin-induced liquid secretion by porcine bronchi. American Journal of Physiology: Lung Cellular and Molecular Physiology, 290(1), L97-L104. http://ajplung.physiology.org/content/290/1/L97.full