Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores

Emma King, Najma Rachidi, Nicholas Morrice, Kevin G. Hardwick, Michael J. R. Stark

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate kinetochore–microtubule attachments and for the response to lack of tension between sister kinetochores. Here we identify residues in the checkpoint protein Mad3p that are phosphorylated by Ipl1p. When phosphorylation of Mad3p at two sites is prevented, the cell’s response to reduced kinetochore tension is dramatically curtailed. Our data provide strong evidence for a distinct checkpoint pathway responding to lack of sister kinetochore tension, in which Ipl1p-dependent phosphorylation of Mad3p is a key step.
    Original languageEnglish
    Pages (from-to)1163-1168
    Number of pages6
    JournalGenes & Development
    Volume21
    Issue number10
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Mad3
    • Aurora kinase
    • Spindle checkpoint

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