Shewanella electrodiphila sp. nov., a psychrotolerant bacterium isolated from Mid-Atlantic Ridge deep-sea sediments

Jinwei Zhang, J. Grant Burgess (Lead / Corresponding author)

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Strains MAR441T and MAR445 were isolated from Mid-Atlantic Ridge (MAR) sediments from a depth of 2,734 m, and found to belong to the genus Shewanella. The strains were rod shaped, pigmented, non-motile, and capable of anaerobic growth either by fermentation of carbohydrates or by anaerobic respiration. The strains utilized a variety of electron acceptors, including nitrate and ferric compounds and could utilize peptone when grown anaerobically in a two-chambered microbial fuel cell (MFC), which use carbon cloth electrodes and deliver a stable power output of ~150-200 mW/m2. The major fatty acids were typical of the genus Shewanella, with major components of C13:0, iso-C13:0, iso-C15:0, C16:0, C16:1ω7c, C18:1ω7c and C20:5ω3 fatty acids. The DNA G+C content of strains MAR441T and MAR445 were 42.4 mol %. 16S rRNA gene sequence analysis indicated that strains MAR441T and MAR445 were most closely related to Shewanella olleyana (sequence similarities 97.9 %). DNA-DNA hybridization demonstrated only 15.6-37.2 % relatedness between strain MAR441T and the type strains of related Shewanella species. Phenotypic characteristics confirmed that these isolates constituted a novel species of the genus Shewanella. The type strain of Shewanella electrodiphila is MAR441T (=ATCC BAA-2408 T =DSM24955 T).

Original languageEnglish
Pages (from-to)2882-2889
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number9
Early online date21 May 2015
DOIs
Publication statusPublished - Sep 2015

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