Isolation, analysis and marker utility of novel miniature inverted repeat transposable elements from the barley genome

Maura Lyons, Linda Cardle, Nils Rostoks, Robbie Waugh, Andrew J. Flavell (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Four previously undescribed families of miniature inverted repeat transposable elements (MITEs) were isolated by searching barley genomic DNA using structure-based criteria. Putative MITEs were confirmed by PCR to determine their insertional polymorphism in a panel of diverse barley germplasm. Copy numbers for all these familes are somewhat low (less than 1,000 copies per family per haploid genome). In contrast to previous studies, a higher proportion of insertions of the new MITEs are found within known transposable elements (27%) than are associated with genes (15%). Preliminary studies were conducted on two of the new MITE families to test their utility as molecular markers. Insertional polymorphism levels for both the families are high and diversity trees produced by both the families are similar and congruent with known relationships among the germplasm studied, suggesting that both the MITE families are useful markers of barley genetic diversity.

    Original languageEnglish
    Pages (from-to)275-285
    Number of pages11
    JournalMolecular Genetics and Genomics
    Volume280
    Issue number4
    DOIs
    Publication statusPublished - Oct 2008

    Keywords

    • barley
    • genetic diversity
    • transposable element
    • SSAP
    • MITE
    • FAMILY HEARTBREAKER
    • RESISTANCE GENES
    • HORDEUM-VULGARE
    • DNA TRANSPOSON
    • MAIZE
    • RICE
    • TOURIST
    • L.
    • DIFFERENTIATION
    • IDENTIFICATION

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