Isolation and purification of protoplasts from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans

C. White, G. M. Gadd (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Novozym 234, a commercially available enzyme, has been used successfully to prepare protoplasts from yeast-like cells, mycelium and chlamydospores of the polymorphic fungus Aureobasidium pullulans. Use of the density gradient material Percoll after wall digestion resulted in preparations of high purity that were metabolically active. Size distributions of protoplasts were determined easily using Coulter analysis.
    Original languageEnglish
    Pages (from-to)1031-1034
    Number of pages4
    JournalJournal of General Microbiology
    Volume130
    Issue number5
    DOIs
    Publication statusPublished - 1 May 1984

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