Isolation and purification of protoplasts from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans

C. White; G. M. Gadd

doi:10.1099/00221287-130-5-1031

Isolation and purification of protoplasts from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans

C. White, G. M. Gadd (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Novozym 234, a commercially available enzyme, has been used successfully to prepare protoplasts from yeast-like cells, mycelium and chlamydospores of the polymorphic fungus Aureobasidium pullulans. Use of the density gradient material Percoll after wall digestion resulted in preparations of high purity that were metabolically active. Size distributions of protoplasts were determined easily using Coulter analysis.

Original language	English
Pages (from-to)	1031-1034
Number of pages	4
Journal	Journal of General Microbiology
Volume	130
Issue number	5
DOIs	https://doi.org/10.1099/00221287-130-5-1031
Publication status	Published - 1 May 1984

ASJC Scopus subject areas

Microbiology

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10.1099/00221287-130-5-1031

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abstract = "Novozym 234, a commercially available enzyme, has been used successfully to prepare protoplasts from yeast-like cells, mycelium and chlamydospores of the polymorphic fungus Aureobasidium pullulans. Use of the density gradient material Percoll after wall digestion resulted in preparations of high purity that were metabolically active. Size distributions of protoplasts were determined easily using Coulter analysis.",

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AB - Novozym 234, a commercially available enzyme, has been used successfully to prepare protoplasts from yeast-like cells, mycelium and chlamydospores of the polymorphic fungus Aureobasidium pullulans. Use of the density gradient material Percoll after wall digestion resulted in preparations of high purity that were metabolically active. Size distributions of protoplasts were determined easily using Coulter analysis.

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Isolation and purification of protoplasts from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans

Abstract

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