Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit

Homology with protein phosphatase 2A

Norbert Berndt, David G. Campbell, F. Barry Caudwell, Philip Cohen, Edgar F. da Cruz e Silva, Odete B. da Cruz e Silva, Patricia T.W. Cohen

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalFEBS Letters
Volume223
Issue number2
DOIs
Publication statusPublished - 2 Nov 1987

Fingerprint

Protein Phosphatase 1
Protein Phosphatase 2
Sequence Analysis
Complementary DNA
Clone Cells
Molecular mass
Gene Library
Northern Blotting
Alkaline Phosphatase
Muscle
Skeletal Muscle
Rabbits
Amino Acids
Messenger RNA
Proteins

Keywords

  • Amino acid sequence homology
  • cDNA cloning
  • Nucleotide sequence
  • Protein phosphatase

Cite this

Berndt, Norbert ; Campbell, David G. ; Caudwell, F. Barry ; Cohen, Philip ; da Cruz e Silva, Edgar F. ; da Cruz e Silva, Odete B. ; Cohen, Patricia T.W. / Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit : Homology with protein phosphatase 2A. In: FEBS Letters. 1987 ; Vol. 223, No. 2. pp. 340-346.
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abstract = "A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49{\%} of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21{\%} sequence identity with a region of mammalian alkaline phosphatases.",
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Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit : Homology with protein phosphatase 2A. / Berndt, Norbert; Campbell, David G.; Caudwell, F. Barry; Cohen, Philip; da Cruz e Silva, Edgar F.; da Cruz e Silva, Odete B.; Cohen, Patricia T.W.

In: FEBS Letters, Vol. 223, No. 2, 02.11.1987, p. 340-346.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit

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AU - Berndt, Norbert

AU - Campbell, David G.

AU - Caudwell, F. Barry

AU - Cohen, Philip

AU - da Cruz e Silva, Edgar F.

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AU - Cohen, Patricia T.W.

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AB - A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.

KW - Amino acid sequence homology

KW - cDNA cloning

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