Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A

Norbert Berndt; David G. Campbell; F. Barry Caudwell; Philip Cohen; Edgar F. da Cruz e Silva; Odete B. da Cruz e Silva; Patricia T.W. Cohen

doi:10.1016/0014-5793(87)80316-2

Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A

Norbert Berndt
, David G. Campbell
, F. Barry Caudwell
, Philip Cohen
, Edgar F. da Cruz e Silva
, Odete B. da Cruz e Silva
, Patricia T.W. Cohen

MRC PPU

Research output: Contribution to journal › Article › peer-review

114 Citations (Scopus)

Abstract

A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.

Original language	English
Pages (from-to)	340-346
Number of pages	7
Journal	FEBS Letters
Volume	223
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(87)80316-2
Publication status	Published - 2 Nov 1987

Keywords

Amino acid sequence homology
cDNA cloning
Nucleotide sequence
Protein phosphatase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Cite this

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title = "Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A",

abstract = "A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49\% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21\% sequence identity with a region of mammalian alkaline phosphatases.",

keywords = "Amino acid sequence homology, cDNA cloning, Nucleotide sequence, Protein phosphatase",

author = "Norbert Berndt and Campbell, \{David G.\} and Caudwell, \{F. Barry\} and Philip Cohen and \{da Cruz e Silva\}, \{Edgar F.\} and \{da Cruz e Silva\}, \{Odete B.\} and Cohen, \{Patricia T.W.\}",

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doi = "10.1016/0014-5793(87)80316-2",

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T2 - Homology with protein phosphatase 2A

AU - Berndt, Norbert

AU - Campbell, David G.

AU - Caudwell, F. Barry

AU - Cohen, Philip

AU - da Cruz e Silva, Edgar F.

AU - da Cruz e Silva, Odete B.

AU - Cohen, Patricia T.W.

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AB - A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.

KW - Amino acid sequence homology

KW - cDNA cloning

KW - Nucleotide sequence

KW - Protein phosphatase

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Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A

Abstract

Keywords

ASJC Scopus subject areas

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