Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2

E. A. SHEPHARD; I. R. PHILLIPS; I. SANTISTEBAN; L. F. WEST; C. N.A. PALMER; A. ASHWORTH; S. POVEY

doi:10.1111/j.1469-1809.1989.tb01798.x

Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2

E. A. SHEPHARD, I. R. PHILLIPS, I. SANTISTEBAN, L. F. WEST, C. N.A. PALMER, A. ASHWORTH, S. POVEY

Population Health and Genomics

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

We have isolated and sequenced cDNA clones that code for rat and human NADPH dependent cytochrome P‐450 reductase. The cDNA coding for the human protein was used to analyse, by Southern blot hybridization, DNA isolated from a panel of 8 independent humanrodent somatic cell hybrids. The results indicate that cytochrome P‐450 reductase is encoded by a single gene (POR) located on human chromosome 7(pter‐q22). Analysis of human metaphase chromosomes by hybridization in situ confirmed the results and refined the localization to 7q11.2. Northern blot hybridization revealed that in human liver the expression of the gene varies by less than 3‐fold between different individuals.

Original language	English
Pages (from-to)	291-301
Number of pages	11
Journal	Annals of Human Genetics
Volume	53
Issue number	4
DOIs	https://doi.org/10.1111/j.1469-1809.1989.tb01798.x
Publication status	Published - Oct 1989

ASJC Scopus subject areas

Genetics
Genetics(clinical)

Access to Document

10.1111/j.1469-1809.1989.tb01798.x

Cite this

@article{43db274996474c10ad2999568ded2368,

title = "Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2",

abstract = "We have isolated and sequenced cDNA clones that code for rat and human NADPH dependent cytochrome P‐450 reductase. The cDNA coding for the human protein was used to analyse, by Southern blot hybridization, DNA isolated from a panel of 8 independent humanrodent somatic cell hybrids. The results indicate that cytochrome P‐450 reductase is encoded by a single gene (POR) located on human chromosome 7(pter‐q22). Analysis of human metaphase chromosomes by hybridization in situ confirmed the results and refined the localization to 7q11.2. Northern blot hybridization revealed that in human liver the expression of the gene varies by less than 3‐fold between different individuals.",

author = "SHEPHARD, {E. A.} and PHILLIPS, {I. R.} and I. SANTISTEBAN and WEST, {L. F.} and PALMER, {C. N.A.} and A. ASHWORTH and S. POVEY",

year = "1989",

month = oct,

doi = "10.1111/j.1469-1809.1989.tb01798.x",

language = "English",

volume = "53",

pages = "291--301",

journal = "Annals of Human Genetics",

issn = "0003-4800",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2

AU - SHEPHARD, E. A.

AU - PHILLIPS, I. R.

AU - SANTISTEBAN, I.

AU - WEST, L. F.

AU - PALMER, C. N.A.

AU - ASHWORTH, A.

AU - POVEY, S.

PY - 1989/10

Y1 - 1989/10

N2 - We have isolated and sequenced cDNA clones that code for rat and human NADPH dependent cytochrome P‐450 reductase. The cDNA coding for the human protein was used to analyse, by Southern blot hybridization, DNA isolated from a panel of 8 independent humanrodent somatic cell hybrids. The results indicate that cytochrome P‐450 reductase is encoded by a single gene (POR) located on human chromosome 7(pter‐q22). Analysis of human metaphase chromosomes by hybridization in situ confirmed the results and refined the localization to 7q11.2. Northern blot hybridization revealed that in human liver the expression of the gene varies by less than 3‐fold between different individuals.

AB - We have isolated and sequenced cDNA clones that code for rat and human NADPH dependent cytochrome P‐450 reductase. The cDNA coding for the human protein was used to analyse, by Southern blot hybridization, DNA isolated from a panel of 8 independent humanrodent somatic cell hybrids. The results indicate that cytochrome P‐450 reductase is encoded by a single gene (POR) located on human chromosome 7(pter‐q22). Analysis of human metaphase chromosomes by hybridization in situ confirmed the results and refined the localization to 7q11.2. Northern blot hybridization revealed that in human liver the expression of the gene varies by less than 3‐fold between different individuals.

UR - http://www.scopus.com/inward/record.url?scp=0024447464&partnerID=8YFLogxK

U2 - 10.1111/j.1469-1809.1989.tb01798.x

DO - 10.1111/j.1469-1809.1989.tb01798.x

M3 - Article

C2 - 2516426

AN - SCOPUS:0024447464

SN - 0003-4800

VL - 53

SP - 291

EP - 301

JO - Annals of Human Genetics

JF - Annals of Human Genetics

IS - 4

ER -

Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this