Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2

E. A. SHEPHARD, I. R. PHILLIPS, I. SANTISTEBAN, L. F. WEST, C. N.A. PALMER, A. ASHWORTH, S. POVEY

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We have isolated and sequenced cDNA clones that code for rat and human NADPH dependent cytochrome P‐450 reductase. The cDNA coding for the human protein was used to analyse, by Southern blot hybridization, DNA isolated from a panel of 8 independent humanrodent somatic cell hybrids. The results indicate that cytochrome P‐450 reductase is encoded by a single gene (POR) located on human chromosome 7(pter‐q22). Analysis of human metaphase chromosomes by hybridization in situ confirmed the results and refined the localization to 7q11.2. Northern blot hybridization revealed that in human liver the expression of the gene varies by less than 3‐fold between different individuals.

Original languageEnglish
Pages (from-to)291-301
Number of pages11
JournalAnnals of Human Genetics
Volume53
Issue number4
DOIs
Publication statusPublished - Oct 1989

Fingerprint Dive into the research topics of 'Isolation of a human cytochrome P‐450 reductase cDNA clone and localization of the corresponding gene to chromosome 7q11.2'. Together they form a unique fingerprint.

  • Cite this