Isolation of NEDDylated proteins in human cells

Orsolya Leidecker, Dimitris P. Xirodimas

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    4 Citations (Scopus)

    Abstract

    Identification of substrates for post-translational modification with ubiquitin and ubiquitin-like molecules is a key in our understanding of the role of this modification in the regulation of biological processes. The ubiquitin-like molecule NEDD8 plays a vital role in cell growth, viability, and development. In this chapter, we describe a method for efficient isolation and detection of NEDDylated proteins in human cells.
    Original languageEnglish
    Title of host publicationUbiquitin family modifiers and the proteasome
    Subtitle of host publicationreviews and protocols
    EditorsR. Jürgen Dohmen, Martin Scheffner
    PublisherHumana Press
    Pages133-140
    Number of pages8
    ISBN (Electronic)9781617794742
    ISBN (Print)9781617794735
    DOIs
    Publication statusPublished - 2012

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume832
    ISSN (Print)1064-3745

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