Isolation of two Kocuria species capable of growing on various polycyclic aromatic hydrocarbons

Rifat Zubair Ahmed, Nuzhat Ahmed, Geoffrey Michael Gadd

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    Abstract

    Different samples collected from crude oil contaminated beach were enriched for isolation of bacterial strains capable of growing on naphthalene, phenanthrene and fluoranthene. Respiratory reduction of WST-1{4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate} to a colored formazan showed that one isolated strain CMG2028, identified as Kocuria flava by 16s rRNA, grew on naphthalene and phenanthrene while CMG2042, identified as Kocuria rosea grew on all three polycyclic aromatic hydrocarbons (PAHs). In naphthalene degradation test, 64 and 47% residual naphthalene was extracted after ten days of incubation from culture medium of K. rosea CMG2042 and K. flava CMG2028, respectively, when provided with 0.5 mg ml(-1) concentration as sole carbon source. Due to addition of 0.5 mg ml(-1) yeast extract as additional carbon source, residual naphthalene extracted was 41 and 55% from K. rosea CMG2042 and K. flava CMG2028, respectively. Both strains exhibited growth on 0.01 mg ml(-1) phenanthrene and fluoranthene in yeast extract added or omitted medium but only K. rosea CMG2042 degraded 9% phenanthrene as a sole carbon source. Both strains had growth on minimal agar plates coated with Iranian light crude oil as sole carbon source and on agar medium added with yeast extract.

    Original languageEnglish
    Pages (from-to)3611-3617
    Number of pages7
    JournalAfrican Journal of Biotechnology
    Volume9
    Issue number24
    Publication statusPublished - 14 Jun 2010

    Keywords

    • Biodegradation
    • crude oil
    • Kocuria
    • polycyclic aromatic hydrocarbons
    • yeast extract
    • PHYLOGENETIC DIVERSITY
    • MANGROVE SEDIMENT
    • PAH DEGRADATION
    • BACTERIA
    • BIODEGRADATION
    • GROWTH
    • SOIL
    • OPTIMIZATION
    • PHENANTHRENE
    • CONSORTIUM

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