Isolation of U5 snRNP particles.

G. Pironcheva, N. Zhelev, G. Russev

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A method for the isolation of intact U5 small nuclear RNP particles from HeLa cells has been developed. The procedure includes nuclear extraction of the particles at moderate ionic strength, fractionation in agarose gels, electrophoretic transfer on DEAE cellulose paper and elution with ammonium chloride. The purified U5 snRNP particles contain U5 RNA and a set of eleven proteins. They retain their antigenicity as judged by their reaction with autoantibodies from patients with connective tissue diseases.

    Original languageEnglish
    Pages (from-to)39-44
    Number of pages6
    JournalCytobios
    Volume56
    Issue number224
    Publication statusPublished - 1988

    ASJC Scopus subject areas

    • Aquatic Science
    • Cell Biology

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