K-channels in an insulin-secreting cell line: Effects of ATP and sulphonylureas

M. L. J. Ashford, N. C. Sturgess, D. L. Cook, C. N. Hales

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    9 Citations (Scopus)

    Abstract

    The recent application of the patch-clamp technique to the study of single channels present in the ß-cell plasma membrane has led to the discovery of a channel the activity of which is inhibited by the application of ATP to the membrane cytoplasmic surface7. It has been suggested that this channel may be responsible for glucose-induced ß-cell depolarisation and that ATP may be the long-sought connection between glucose metabolism and the excitation of insulin secretion7,8. Cell lines are proving to be very convenient and reproducible systems with which to study metabolic processes and their regulation and we have found them particularly suitable for patch clamp procedures. We have therefore investigated a new insulin-secreting cell line5 using the patch-clamp technique in order to study potassium channels and their role in the control of insulin secretion.
    Original languageEnglish
    Title of host publicationBiophysics of the pancreatic ß-cell
    EditorsIllani Atwater, Eduardo Rojas, Bernat Soria
    PublisherPlenum Press
    Pages69-76
    Number of pages8
    ISBN (Electronic)9781468453140
    ISBN (Print)9781468453164
    DOIs
    Publication statusPublished - 1986

    Publication series

    NameAdvances in Experimental Medicine and Biology
    PublisherSpringer US
    Volume211
    ISSN (Print)0065-2598

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    Cite this

    Ashford, M. L. J., Sturgess, N. C., Cook, D. L., & Hales, C. N. (1986). K-channels in an insulin-secreting cell line: Effects of ATP and sulphonylureas. In I. Atwater, E. Rojas, & B. Soria (Eds.), Biophysics of the pancreatic ß-cell (pp. 69-76). (Advances in Experimental Medicine and Biology; Vol. 211). Plenum Press. https://doi.org/10.1007/978-1-4684-5314-0_6