Projects per year
Abstract
Hippocampal sclerosis is a common acquired disease that is a major cause of drug-resistant epilepsy. A mechanism that has been proposed to lead from brain insult to hippocampal sclerosis is the excessive generation of reactive oxygen species, and consequent mitochondrial failure. Here we use a novel strategy to increase endogenous antioxidant defences using RTA 408, which we show activates nuclear factor erythroid 2-related factor 2 (Nrf2, encoded by NFE2L2) through inhibition of kelch like ECH associated protein 1 (KEAP1) through its primary sensor C151. Activation of Nrf2 with RTA 408 inhibited reactive oxygen species production, mitochondrial depolarization and cell death in an in vitro model of seizure-like activity. RTA 408 given after status epilepticus in vivo increased ATP, prevented neuronal death, and dramatically reduced (by 94%) the frequency of late spontaneous seizures for at least 4 months following status epilepticus. Thus, acute KEAP1 inhibition following status epilepticus exerts a neuroprotective and disease-modifying effect, supporting the hypothesis that reactive oxygen species generation is a key event in the development of epilepsy.
Original language | English |
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Pages (from-to) | 1390-1403 |
Number of pages | 14 |
Journal | Brain |
Volume | 141 |
Issue number | 5 |
Early online date | 12 Mar 2018 |
DOIs | |
Publication status | Published - May 2018 |
Keywords
- epilepsy
- epileptogenesis
- mitochondrial dysfunction
- Nrf2-KEAP1 pathway
- oxidative stress
ASJC Scopus subject areas
- Clinical Neurology
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Dive into the research topics of 'KEAP1 inhibition is neuroprotective and suppresses the development of epilepsy'. Together they form a unique fingerprint.Projects
- 1 Finished
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The Spatiotemporal Regulation of the Keap1/Nrf2 Pathway (Joint with University College London)
Dinkova-Kostova, A. (Investigator)
Biotechnology and Biological Sciences Research Council
30/09/14 → 27/02/18
Project: Research