KESTREL: A powerful method for identifying the physiological substrates of protein kinases

Philip Cohen, Axel Knebelt

    Research output: Contribution to journalReview article

    62 Citations (Scopus)

    Abstract

    The identification of all the substrates of every protein kinase is one of the major challenges of post-genomic research. Here we review a powerful method for tackling this problem that we have developed over the last 5 years. The method has so far been used to identify novel substrates for eight different protein kinases, demonstrating that it is of general utility. Importantly, the method can be used to identify distinct physiological substrates of protein kinases, such as PKB (protein kinase B) and SGK (serum- and glucocorticoid- induced kinase), that are closely related in structure and have similar specificity determinants.

    Original languageEnglish
    Pages (from-to)1-6
    Number of pages6
    JournalBiochemical Journal
    Volume393
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2006

    Keywords

    • Glycogen synthase kinase 3 (GSK3)
    • Kinase substrate tracking and elucidation (KESTREL)
    • Mitogen-activated-protein-kinase-activated protein kinase-2 (MAPKAP-K2)
    • Protein kinase B (PKB)
    • Serum- and glucocorticoid-induced protein kinase (SGK)

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