Kinetochore-microtubule error correction is driven by differentially regulated interaction modes

Maria Kalantzaki, Etsushi Kitamura, Tongli Zhang, Akihisa Mino, Béla Novák , Tomoyuki U. Tanaka (Lead / Corresponding author)

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    32 Citations (Scopus)
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    Abstract

    For proper chromosome segregation, sister kinetochores must interact with microtubules from opposite spindle poles (bi-orientation). To establish bi-orientation, aberrant kinetochore–microtubule attachments are disrupted (error correction) by ​aurora B kinase (​Ipl1 in budding yeast). Paradoxically, during this disruption, new attachments are still formed efficiently to enable fresh attempts at bi-orientation. How this is possible remains an enigma. Here we show that kinetochore attachment to the microtubule lattice (lateral attachment) is impervious to ​aurora B regulation, but attachment to the microtubule plus end (end-on attachment) is disrupted by this kinase. Thus, a new lateral attachment is formed without interference, then converted to end-on attachment and released if incorrect. This process continues until bi-orientation is established and stabilized by tension across sister kinetochores. We reveal how ​aurora B specifically promotes disruption of the end-on attachment through phospho-regulation of kinetochore components Dam1 and Ndc80. Our results reveal fundamental mechanisms for promoting error correction for bi-orientation.
    Original languageEnglish
    Pages (from-to)421-433
    Number of pages13
    JournalNature Cell Biology
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - Apr 2015

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