Projects per year
For proper chromosome segregation, sister kinetochores must interact with microtubules from opposite spindle poles (bi-orientation). To establish bi-orientation, aberrant kinetochore–microtubule attachments are disrupted (error correction) by aurora B kinase (Ipl1 in budding yeast). Paradoxically, during this disruption, new attachments are still formed efficiently to enable fresh attempts at bi-orientation. How this is possible remains an enigma. Here we show that kinetochore attachment to the microtubule lattice (lateral attachment) is impervious to aurora B regulation, but attachment to the microtubule plus end (end-on attachment) is disrupted by this kinase. Thus, a new lateral attachment is formed without interference, then converted to end-on attachment and released if incorrect. This process continues until bi-orientation is established and stabilized by tension across sister kinetochores. We reveal how aurora B specifically promotes disruption of the end-on attachment through phospho-regulation of kinetochore components Dam1 and Ndc80. Our results reveal fundamental mechanisms for promoting error correction for bi-orientation.
|Number of pages||13|
|Journal||Nature Cell Biology|
|Publication status||Published - Apr 2015|
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- 4 Finished
SELFCC: Chromosome Self-clearing Completes Sister Chromatid Separation
COMMISSION OF THE EUROPEAN COMMUNITIES
1/04/13 → 30/09/18
Strategic Award: Wellcome Trust Technology Platform
Blow, J., Lamond, A. & Owen-Hughes, T.
1/01/13 → 30/09/18
Molecular Mechanisms Regulating the Kinetochore-Microtubule Interaction in Mitosis (Principal Research Fellowship)
1/04/12 → 30/04/21
- Gene Regulation and Expression - Professor of Cell and Molecular Biology