Kinetochore-microtubule interaction during S phase in Saccharomyces cerevisiae

Etsushi Kitamura, Kozo Tanaka, Yoko Kitamura, Tomoyuki U. Tanaka

    Research output: Contribution to journalArticle

    82 Citations (Scopus)

    Abstract

    In the budding yeast Saccharomyces cerevisiae, microtubule-organizing centers called spindle pole bodies (SPBs) are embedded in the nuclear envelope, which remains intact throughout the cell cycle (closed mitosis). Kinetochores are tethered to SPBs by microtubules during most of the cell cycle, including G1 and M phases; however, it has been a topic of debate whether microtubule interaction is constantly maintained or transiently disrupted during chromosome duplication. Here, we show that centromeres are detached from microtubules for 1-2 min and displaced away from a spindle pole in early S phase. These detachment and displacement events are caused by centromere DNA replication, which results in disassembly of kinetochores. Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are subsequently transported poleward by microtubules. Our study gives new insights into kinetochore-microtubule interaction and kinetochore duplication during S phase in a closed mitosis.

    Original languageEnglish
    Pages (from-to)3319-3330
    Number of pages12
    JournalGenes & Development
    Volume21
    Issue number24
    DOIs
    Publication statusPublished - 15 Dec 2007

    Keywords

    • kinetochore
    • microtubule
    • S phase
    • Saccharomyces cerevisiae
    • closed mitosis
    • SPINDLE POLE BODY
    • CHROMOSOME BI-ORIENTATION
    • BUDDING YEAST
    • MITOTIC SPINDLE
    • DNA-REPLICATION
    • CENTROSOME DUPLICATION
    • PRECOCIOUS SEPARATION
    • MOLECULAR-MECHANISMS
    • PROTEIN
    • ANAPHASE

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