Kinetochore-microtubule interactions

Lesley Clayton, Tomoyuki U. Tanaka

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Kinetochores are the multiprotein macromolecular assemblies on chromatin that ensure the accurate and timely segregation of chromosomes at during mitosis. To achieve this, kinetochores must interact with the microtubules of the spindle and microtubule-associated proteins. The nature of the kinetochore-microtubule interaction varies during the stages of the mitotic cycle, starting with initial capture and progressing through bi-orientation and congression at prometaphase/ metaphase, then finally separation of sister kinetochores/chromatids during anaphase. All the while during this process, kinetochores are able to signal their state of microtubule binding to the cell cycle control machinery. They are also able to influence microtubule dynamics in order to achieve chromosome segregation. Determining the structure and biochemistry of these various interactions continues to be a major objective of research in this field. Much of the cell biology/cytology of cell division has originally been described in metazoan cells in culture, e.g. PtKcells, newt lung, mouse and human cell lines etc., as their relatively large size and flat growth characteristics in culture make them easy to work with. However recent advances in microscopy, particularly fluorescence techniques, have made it possible to visualise spindle components in living cells of both budding and fission yeast, and also Drosophila cells, all of which had previously proven too small to image successfully, but which have powerful advantages in terms of genetics and proteomics. A great deal of research on kinetochore structure and function has been performed using the budding yeast Saccharomyces cerevisiae as an experimental system. Many of the proteins that make up the yeast kinetochore have counterparts in other organisms, including mammals (McAinsh et al. 2003). In addition, the yeast centromere DNA spans only about 130 bp (Hegemann and Fleig 1993), and a kinetochore interacts with a single microtubule in metaphase, compared with dozens in higher organisms (Winey et al. 1995; OToole et al. 1999). These features, coupled with the ease of genetic manipulation and the extensive proteomic information available have meant that much progress has been made in studying the yeast kinetochore. This chapter will examine, and compare where possible, the various kinds of interactions that occur between kinetochores and microtubules at different times during mitosis, both in yeast and in higher organisms.
    Original languageEnglish
    Title of host publicationThe kinetochore
    Subtitle of host publicationfrom molecular discoveries to cancer therapy
    EditorsPeter De Wulf , William C. Earnshaw
    Place of PublicationNew York
    PublisherSpringer
    Pages269-292
    Number of pages24
    ISBN (Electronic)9780387690766
    ISBN (Print)9780387690735
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    kinetochores
    microtubules
    yeasts
    chromosome segregation
    metaphase
    cell biology
    proteomics
    mitosis
    organisms
    chromatids
    human cell lines
    Schizosaccharomyces pombe
    anaphase
    centromeres
    fluorescence microscopy
    genetic engineering
    salamanders and newts
    biochemistry
    chromatin
    cell division

    Cite this

    Clayton, L., & Tanaka, T. U. (2009). Kinetochore-microtubule interactions. In P. De Wulf , & W. C. Earnshaw (Eds.), The kinetochore: from molecular discoveries to cancer therapy (pp. 269-292). New York: Springer . https://doi.org/10.1007/978-0-387-69076-6_9
    Clayton, Lesley ; Tanaka, Tomoyuki U. / Kinetochore-microtubule interactions. The kinetochore: from molecular discoveries to cancer therapy. editor / Peter De Wulf ; William C. Earnshaw . New York : Springer , 2009. pp. 269-292
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    abstract = "Kinetochores are the multiprotein macromolecular assemblies on chromatin that ensure the accurate and timely segregation of chromosomes at during mitosis. To achieve this, kinetochores must interact with the microtubules of the spindle and microtubule-associated proteins. The nature of the kinetochore-microtubule interaction varies during the stages of the mitotic cycle, starting with initial capture and progressing through bi-orientation and congression at prometaphase/ metaphase, then finally separation of sister kinetochores/chromatids during anaphase. All the while during this process, kinetochores are able to signal their state of microtubule binding to the cell cycle control machinery. They are also able to influence microtubule dynamics in order to achieve chromosome segregation. Determining the structure and biochemistry of these various interactions continues to be a major objective of research in this field. Much of the cell biology/cytology of cell division has originally been described in metazoan cells in culture, e.g. PtKcells, newt lung, mouse and human cell lines etc., as their relatively large size and flat growth characteristics in culture make them easy to work with. However recent advances in microscopy, particularly fluorescence techniques, have made it possible to visualise spindle components in living cells of both budding and fission yeast, and also Drosophila cells, all of which had previously proven too small to image successfully, but which have powerful advantages in terms of genetics and proteomics. A great deal of research on kinetochore structure and function has been performed using the budding yeast Saccharomyces cerevisiae as an experimental system. Many of the proteins that make up the yeast kinetochore have counterparts in other organisms, including mammals (McAinsh et al. 2003). In addition, the yeast centromere DNA spans only about 130 bp (Hegemann and Fleig 1993), and a kinetochore interacts with a single microtubule in metaphase, compared with dozens in higher organisms (Winey et al. 1995; OToole et al. 1999). These features, coupled with the ease of genetic manipulation and the extensive proteomic information available have meant that much progress has been made in studying the yeast kinetochore. This chapter will examine, and compare where possible, the various kinds of interactions that occur between kinetochores and microtubules at different times during mitosis, both in yeast and in higher organisms.",
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    Clayton, L & Tanaka, TU 2009, Kinetochore-microtubule interactions. in P De Wulf & WC Earnshaw (eds), The kinetochore: from molecular discoveries to cancer therapy. Springer , New York, pp. 269-292. https://doi.org/10.1007/978-0-387-69076-6_9

    Kinetochore-microtubule interactions. / Clayton, Lesley; Tanaka, Tomoyuki U.

    The kinetochore: from molecular discoveries to cancer therapy. ed. / Peter De Wulf ; William C. Earnshaw . New York : Springer , 2009. p. 269-292.

    Research output: Chapter in Book/Report/Conference proceedingChapter

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    AB - Kinetochores are the multiprotein macromolecular assemblies on chromatin that ensure the accurate and timely segregation of chromosomes at during mitosis. To achieve this, kinetochores must interact with the microtubules of the spindle and microtubule-associated proteins. The nature of the kinetochore-microtubule interaction varies during the stages of the mitotic cycle, starting with initial capture and progressing through bi-orientation and congression at prometaphase/ metaphase, then finally separation of sister kinetochores/chromatids during anaphase. All the while during this process, kinetochores are able to signal their state of microtubule binding to the cell cycle control machinery. They are also able to influence microtubule dynamics in order to achieve chromosome segregation. Determining the structure and biochemistry of these various interactions continues to be a major objective of research in this field. Much of the cell biology/cytology of cell division has originally been described in metazoan cells in culture, e.g. PtKcells, newt lung, mouse and human cell lines etc., as their relatively large size and flat growth characteristics in culture make them easy to work with. However recent advances in microscopy, particularly fluorescence techniques, have made it possible to visualise spindle components in living cells of both budding and fission yeast, and also Drosophila cells, all of which had previously proven too small to image successfully, but which have powerful advantages in terms of genetics and proteomics. A great deal of research on kinetochore structure and function has been performed using the budding yeast Saccharomyces cerevisiae as an experimental system. Many of the proteins that make up the yeast kinetochore have counterparts in other organisms, including mammals (McAinsh et al. 2003). In addition, the yeast centromere DNA spans only about 130 bp (Hegemann and Fleig 1993), and a kinetochore interacts with a single microtubule in metaphase, compared with dozens in higher organisms (Winey et al. 1995; OToole et al. 1999). These features, coupled with the ease of genetic manipulation and the extensive proteomic information available have meant that much progress has been made in studying the yeast kinetochore. This chapter will examine, and compare where possible, the various kinds of interactions that occur between kinetochores and microtubules at different times during mitosis, both in yeast and in higher organisms.

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    Clayton L, Tanaka TU. Kinetochore-microtubule interactions. In De Wulf P, Earnshaw WC, editors, The kinetochore: from molecular discoveries to cancer therapy. New York: Springer . 2009. p. 269-292 https://doi.org/10.1007/978-0-387-69076-6_9