Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I

Kirsten P. Rabitsch, Mark Petronczki, Jean-Paul Javerzat, Sylvie Genier, Barbara Chwalla, Alex Schleiffer, Tomoyuki U. Tanaka, Kim Nasmyth

    Research output: Contribution to journalArticlepeer-review

    158 Citations (Scopus)

    Abstract

    Halving of the chromosome number during meiosis I depends on the segregation of maternal and paternal centromeres. This process relies on the attachment of sister centromeres to microtubules emanating from the same spindle pole. We describe here the identification of a protein complex, Csm1/Lrs4, that is essential for monoorientation of sister kinetochores in Saccharomyces cerevisiae. Both proteins are present in vegetative cells, where they reside in the nucleolus. Only shortly before meiosis I do they leave the nucleolus and form a "monopolin" complex with the meiosis-specific Mam1 protein, which binds to kinetochores. Surprisingly, Csm1's homolog in Schizosaccharomyces pombe, Pcs1, is essential for accurate chromosome segregation during mitosis and meiosis II. Csm1 and Pcs1 might clamp together microtubule binding sites on the same (Pcs1) or sister (Csm1) kinetochores.

    Original languageEnglish
    Pages (from-to)535-48
    Number of pages14
    JournalDevelopmental Cell
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - 2003

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