Kinetochores Generate Microtubules with Distal Plus Ends: Their Roles and Limited Lifetime in Mitosis

Etsushi Kitamura, Kozo Tanaka, Shinya Komoto, Yoko Kitamura, Claude Antony, Tomoyuki U. Tanaka (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    56 Citations (Scopus)

    Abstract

    In early mitosis, microtubules can be generated at kinetochores as well as at spindle poles. However, the role and regulation of kinetochore-derived microtubules have been unclear. In general, metaphase spindle microtubules are oriented such that their plus ends bind to kinetochores. However, we now have evidence that, during early mitosis in budding yeast, microtubules are generated at kinetochores with distal plus ends. These kinetochore-derived microtubules interact along their length with microtubules that extend from a spindle pole, facilitating kinetochore loading onto the lateral surface of spindle pole microtubules. Once kinetochores are loaded, microtubules are no longer generated at kinetochores, and those that remain disappear rapidly and do not contribute to the metaphase spindle. Stu2 (the ortholog of vertebrate XMAP215/ch-TOG) localizes to kinetochores and plays a central role in regulating kinetochore-derived microtubules. Our work provides insight into microtubule generation at kinetochores and the mechanisms that facilitate initial kinetochore interaction with spindle pole microtubules.

    Original languageEnglish
    Pages (from-to)248-259
    Number of pages12
    JournalDevelopmental Cell
    Volume18
    Issue number2
    DOIs
    Publication statusPublished - 16 Feb 2010

    Keywords

    • HAMSTER OVARY CELLS
    • MITOTIC-SPINDLE
    • SACCHAROMYCES-CEREVISIAE
    • MOLECULAR-MECHANISMS
    • FIBERS CONTRIBUTES
    • BUDDING YEAST
    • MINUS-END
    • DYNAMICS
    • TUBULIN
    • CHROMOSOMES

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