KATP channels are up-regulated with increasing age in human myometrium

Qingyou Du, Sofija Jovanovic, Lidija Tulic, Dragisa Sljivancanin, David W. Jack, Vojislav Zizic, Khaja Shameem Mohammed Abdul, Ivan Tulic, Aleksandar Jovanovic

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)98-102
    Number of pages5
    JournalMechanisms of Ageing and Development
    Volume134
    Issue number3-4
    DOIs
    Publication statusPublished - 2013

    Keywords

    • MATERNAL AGE
    • Uterus
    • IMPACT
    • MECHANISM
    • PREGNANCY
    • HEART
    • ATP-sensitive K+ channels
    • Partiurent
    • Pregnancy
    • Ageing
    • H9C2 CELLS
    • SENSITIVE POTASSIUM CHANNELS
    • EXPRESSION
    • LABOR
    • GESTATIONAL-AGE

    Cite this

    Du, Q., Jovanovic, S., Tulic, L., Sljivancanin, D., Jack, D. W., Zizic, V., ... Jovanovic, A. (2013). KATP channels are up-regulated with increasing age in human myometrium. Mechanisms of Ageing and Development, 134(3-4), 98-102. https://doi.org/10.1016/j.mad.2013.01.003
    Du, Qingyou ; Jovanovic, Sofija ; Tulic, Lidija ; Sljivancanin, Dragisa ; Jack, David W. ; Zizic, Vojislav ; Mohammed Abdul, Khaja Shameem ; Tulic, Ivan ; Jovanovic, Aleksandar. / KATP channels are up-regulated with increasing age in human myometrium. In: Mechanisms of Ageing and Development. 2013 ; Vol. 134, No. 3-4. pp. 98-102.
    @article{870674ec3f6c4363b8bb59035b4f45e1,
    title = "KATP channels are up-regulated with increasing age in human myometrium",
    abstract = "It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.",
    keywords = "MATERNAL AGE, Uterus, IMPACT, MECHANISM, PREGNANCY, HEART, ATP-sensitive K+ channels, Partiurent, Pregnancy, Ageing, H9C2 CELLS, SENSITIVE POTASSIUM CHANNELS, EXPRESSION, LABOR, GESTATIONAL-AGE",
    author = "Qingyou Du and Sofija Jovanovic and Lidija Tulic and Dragisa Sljivancanin and Jack, {David W.} and Vojislav Zizic and {Mohammed Abdul}, {Khaja Shameem} and Ivan Tulic and Aleksandar Jovanovic",
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    Du, Q, Jovanovic, S, Tulic, L, Sljivancanin, D, Jack, DW, Zizic, V, Mohammed Abdul, KS, Tulic, I & Jovanovic, A 2013, 'KATP channels are up-regulated with increasing age in human myometrium', Mechanisms of Ageing and Development, vol. 134, no. 3-4, pp. 98-102. https://doi.org/10.1016/j.mad.2013.01.003

    KATP channels are up-regulated with increasing age in human myometrium. / Du, Qingyou; Jovanovic, Sofija; Tulic, Lidija; Sljivancanin, Dragisa; Jack, David W.; Zizic, Vojislav; Mohammed Abdul, Khaja Shameem ; Tulic, Ivan; Jovanovic, Aleksandar.

    In: Mechanisms of Ageing and Development, Vol. 134, No. 3-4, 2013, p. 98-102.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - KATP channels are up-regulated with increasing age in human myometrium

    AU - Du, Qingyou

    AU - Jovanovic, Sofija

    AU - Tulic, Lidija

    AU - Sljivancanin, Dragisa

    AU - Jack, David W.

    AU - Zizic, Vojislav

    AU - Mohammed Abdul, Khaja Shameem

    AU - Tulic, Ivan

    AU - Jovanovic, Aleksandar

    PY - 2013

    Y1 - 2013

    N2 - It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.

    AB - It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.

    KW - MATERNAL AGE

    KW - Uterus

    KW - IMPACT

    KW - MECHANISM

    KW - PREGNANCY

    KW - HEART

    KW - ATP-sensitive K+ channels

    KW - Partiurent

    KW - Pregnancy

    KW - Ageing

    KW - H9C2 CELLS

    KW - SENSITIVE POTASSIUM CHANNELS

    KW - EXPRESSION

    KW - LABOR

    KW - GESTATIONAL-AGE

    U2 - 10.1016/j.mad.2013.01.003

    DO - 10.1016/j.mad.2013.01.003

    M3 - Article

    VL - 134

    SP - 98

    EP - 102

    JO - Mechanisms of Ageing and Development

    JF - Mechanisms of Ageing and Development

    SN - 0047-6374

    IS - 3-4

    ER -