KATP channels are up-regulated with increasing age in human myometrium

Qingyou Du, Sofija Jovanovic, Lidija Tulic, Dragisa Sljivancanin, David W. Jack, Vojislav Zizic, Khaja Shameem Mohammed Abdul, Ivan Tulic, Aleksandar Jovanovic

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    Abstract

    It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)98-102
    Number of pages5
    JournalMechanisms of Ageing and Development
    Volume134
    Issue number3-4
    DOIs
    Publication statusPublished - 2013

    Keywords

    • MATERNAL AGE
    • Uterus
    • IMPACT
    • MECHANISM
    • PREGNANCY
    • HEART
    • ATP-sensitive K+ channels
    • Partiurent
    • Pregnancy
    • Ageing
    • H9C2 CELLS
    • SENSITIVE POTASSIUM CHANNELS
    • EXPRESSION
    • LABOR
    • GESTATIONAL-AGE

    Cite this

    Du, Q., Jovanovic, S., Tulic, L., Sljivancanin, D., Jack, D. W., Zizic, V., Mohammed Abdul, K. S., Tulic, I., & Jovanovic, A. (2013). KATP channels are up-regulated with increasing age in human myometrium. Mechanisms of Ageing and Development, 134(3-4), 98-102. https://doi.org/10.1016/j.mad.2013.01.003