TY - JOUR
T1 - KATP channels are up-regulated with increasing age in human myometrium
AU - Du, Qingyou
AU - Jovanovic, Sofija
AU - Tulic, Lidija
AU - Sljivancanin, Dragisa
AU - Jack, David W.
AU - Zizic, Vojislav
AU - Mohammed Abdul, Khaja Shameem
AU - Tulic, Ivan
AU - Jovanovic, Aleksandar
PY - 2013
Y1 - 2013
N2 - It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
AB - It is well established that ageing is associated with decrease in myometrial efficiency and higher incidence of labour complications. In myometrium, the presence of ATP-sensitive K+ (K-ATP) channels has been detected and they could be a factor in regulating uterine quiescence in pregnancy and contractions during labour. Here, we have examined a possibility of ageing-mediated regulation of K-ATP channels in the human myometrium. Myometrial samples were taken from non-pregnant women undergoing hysterectomy (n = 34) and from women undergoing caesarean section in late pregnancy (n = 36). Real time RT-PCR revealed that mRNAs of all known K-ATP channel subunits were present in the human myometrium. In non-pregnant myometrium, ageing up-regulated SUR2B/Kir6.1, subunits forming K-ATP channels in this tissue, without affecting the expression of other channel subunits. In the late pregnant myometrium, the level of subunits that do not form functional K-ATP channels was not affected by age within 20-41 age range. However, uterine SUR2B and Kir6.1 were up-regulated in parturient over 35 years. An ageing-induced increase in those channel subunits was confirmed by Western blotting. Thus, this study suggests that K-ATP channels are up-regulated with increasing age in human myometrium. This may help explain, at least partially, increased rate of birth complications in women aged over 35 years. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
KW - MATERNAL AGE
KW - Uterus
KW - IMPACT
KW - MECHANISM
KW - PREGNANCY
KW - HEART
KW - ATP-sensitive K+ channels
KW - Partiurent
KW - Pregnancy
KW - Ageing
KW - H9C2 CELLS
KW - SENSITIVE POTASSIUM CHANNELS
KW - EXPRESSION
KW - LABOR
KW - GESTATIONAL-AGE
U2 - 10.1016/j.mad.2013.01.003
DO - 10.1016/j.mad.2013.01.003
M3 - Article
C2 - 23369859
SN - 0047-6374
VL - 134
SP - 98
EP - 102
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 3-4
ER -