LAAT-1 is the lysosomal lysine/arginine transporter that maintains amino acid homeostasis

Bin Liu, Hongwei Du, Rachael Rutkowski, Anton Gartner, Xiaochen Wang (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    73 Citations (Scopus)

    Abstract

    Defective catabolite export from lysosomes results in lysosomal storage diseases in humans. Mutations in the cystine transporter gene CTNS cause cystinosis, but other lysosomal amino acid transporters are poorly characterized at the molecular level. Here, we identified the Caenorhabditis elegans lysosomal lysine/arginine transporter LAAT-1. Loss of laat-1 caused accumulation of lysine and arginine in enlarged, degradation-defective lysosomes. In mutants of ctns-1 (C. elegans homolog of CTNS), LAAT-1 was required to reduce lysosomal cystine levels and suppress lysosome enlargement by cysteamine, a drug that alleviates cystinosis by converting cystine to a lysine analog. LAAT-1 also maintained availability of cytosolic lysine/arginine during embryogenesis. Thus, LAAT-1 is the lysosomal lysine/arginine transporter, which suggests a molecular explanation for how cysteamine alleviates a lysosomal storage disease.
    Original languageEnglish
    Pages (from-to)351-354
    Number of pages4
    JournalScience
    Volume337
    Issue number6092
    DOIs
    Publication statusPublished - 2012

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