Laboratory evolution of protein conformational dynamics

Eleanor C Campbell; Galen J Correy; Peter D Mabbitt; Ashley M Buckle; Nobuhiko Tokuriki; Colin J Jackson

doi:10.1016/j.sbi.2017.09.005

Laboratory evolution of protein conformational dynamics

Eleanor C Campbell, Galen J Correy, Peter D Mabbitt, Ashley M Buckle, Nobuhiko Tokuriki, Colin J Jackson

MRC PPU

Research output: Contribution to journal › Review article › peer-review

75 Citations (Scopus)

Abstract

This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.

Original language	English
Pages (from-to)	49-57
Number of pages	9
Journal	Current Opinion in Structural Biology
Volume	50
DOIs	https://doi.org/10.1016/j.sbi.2017.09.005
Publication status	Published - Jun 2018

Keywords

Computer Simulation
Evolution, Molecular
Molecular Dynamics Simulation
Protein Conformation
Protein Engineering
Proteins/chemistry

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10.1016/j.sbi.2017.09.005Licence: Elsevier User Licence

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title = "Laboratory evolution of protein conformational dynamics",

abstract = "This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.",

keywords = "Computer Simulation, Evolution, Molecular, Molecular Dynamics Simulation, Protein Conformation, Protein Engineering, Proteins/chemistry",

author = "Campbell, {Eleanor C} and Correy, {Galen J} and Mabbitt, {Peter D} and Buckle, {Ashley M} and Nobuhiko Tokuriki and Jackson, {Colin J}",

year = "2018",

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doi = "10.1016/j.sbi.2017.09.005",

language = "English",

volume = "50",

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issn = "0959-440X",

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T1 - Laboratory evolution of protein conformational dynamics

AU - Campbell, Eleanor C

AU - Correy, Galen J

AU - Mabbitt, Peter D

AU - Buckle, Ashley M

AU - Tokuriki, Nobuhiko

AU - Jackson, Colin J

PY - 2018/6

Y1 - 2018/6

N2 - This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.

AB - This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.

KW - Computer Simulation

KW - Evolution, Molecular

KW - Molecular Dynamics Simulation

KW - Protein Conformation

KW - Protein Engineering

KW - Proteins/chemistry

U2 - 10.1016/j.sbi.2017.09.005

DO - 10.1016/j.sbi.2017.09.005

M3 - Review article

C2 - 29120734

SN - 0959-440X

VL - 50

SP - 49

EP - 57

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

ER -

Laboratory evolution of protein conformational dynamics

Abstract

Keywords

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