Laboratory evolution of protein conformational dynamics

Eleanor C Campbell, Galen J Correy, Peter D Mabbitt, Ashley M Buckle, Nobuhiko Tokuriki, Colin J Jackson

Research output: Contribution to journalReview article

16 Citations (Scopus)

Abstract

This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.

Original languageEnglish
Pages (from-to)49-57
Number of pages9
JournalCurrent Opinion in Structural Biology
Volume50
DOIs
Publication statusPublished - Jun 2018

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Proteins

Keywords

  • Computer Simulation
  • Evolution, Molecular
  • Molecular Dynamics Simulation
  • Protein Conformation
  • Protein Engineering
  • Proteins/chemistry

Cite this

Campbell, E. C., Correy, G. J., Mabbitt, P. D., Buckle, A. M., Tokuriki, N., & Jackson, C. J. (2018). Laboratory evolution of protein conformational dynamics. Current Opinion in Structural Biology, 50, 49-57. https://doi.org/10.1016/j.sbi.2017.09.005
Campbell, Eleanor C ; Correy, Galen J ; Mabbitt, Peter D ; Buckle, Ashley M ; Tokuriki, Nobuhiko ; Jackson, Colin J. / Laboratory evolution of protein conformational dynamics. In: Current Opinion in Structural Biology. 2018 ; Vol. 50. pp. 49-57.
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Laboratory evolution of protein conformational dynamics. / Campbell, Eleanor C; Correy, Galen J; Mabbitt, Peter D; Buckle, Ashley M; Tokuriki, Nobuhiko; Jackson, Colin J.

In: Current Opinion in Structural Biology, Vol. 50, 06.2018, p. 49-57.

Research output: Contribution to journalReview article

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T1 - Laboratory evolution of protein conformational dynamics

AU - Campbell, Eleanor C

AU - Correy, Galen J

AU - Mabbitt, Peter D

AU - Buckle, Ashley M

AU - Tokuriki, Nobuhiko

AU - Jackson, Colin J

N1 - Copyright © 2017 Elsevier Ltd. All rights reserved.

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N2 - This review focuses on recent work that has begun to establish specific functional roles for protein conformational dynamics, specifically how the conformational landscapes that proteins can sample can evolve under laboratory based evolutionary selection. We discuss recent technical advances in computational and biophysical chemistry, which have provided us with new ways to dissect evolutionary processes. Finally, we offer some perspectives on the emerging view of conformational dynamics and evolution, and the challenges that we face in rationally engineering conformational dynamics.

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KW - Evolution, Molecular

KW - Molecular Dynamics Simulation

KW - Protein Conformation

KW - Protein Engineering

KW - Proteins/chemistry

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DO - 10.1016/j.sbi.2017.09.005

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JO - Current Opinion in Structural Biology

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