Large-scale proteomic analysis of the human spliceosome

Juri Rappsilber, Ursula Ryder, Angus I. Lamond, Matthias Mann

Research output: Contribution to journalReview articlepeer-review

183 Citations (Scopus)

Abstract

In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we now report identification of 311 proteins that copurify with splicing complexes assembled on two separate pre-mRNAs. All known essential human splicing factors were found, and 96 novel proteins were identified, of which 55 contain domains directly linking them to functions in splicing/RNA processing. We also detected 20 proteins related to transcription, which indicates a direct connection between this process and splicing. This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway.

Original languageEnglish
Pages (from-to)1231-1245
Number of pages15
JournalGenome Research
Volume13
Issue number1
DOIs
Publication statusPublished - 2003

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