Length-dependent anisotropic scaling of spindle shape

Sarah Young, Sébastien Besson, Julie P I Welburn (Lead / Corresponding author)

Research output: Contribution to journalArticle

13 Citations (Scopus)
110 Downloads (Pure)

Abstract

Spindle length varies dramatically across species and during early development to segregate chromosomes optimally. Both intrinsic factors, such as regulatory molecules, and extrinsic factors, such as cytoplasmic volume, determine spindle length scaling. However, the properties that govern spindle shape and whether these features can be modulated remain unknown. Here, we analyzed quantitatively how the molecular players which regulate microtubule dynamics control the kinetics of spindle formation and shape. We find that, in absence of Clasp1 and Clasp2, spindle assembly is biphasic due to unopposed inward pulling forces from the kinetochore-fibers and that kinetochore-fibers also alter spindle geometry. We demonstrate that spindle shape scaling is independent of the nature of the molecules that regulate dynamic microtubule properties, but is dependent on the steady-state metaphase spindle length. The shape of the spindle scales anisotropically with increasing length. Our results suggest that intrinsic mechanisms control the shape of the spindle to ensure the efficient capture and alignment of chromosomes independently of spindle length.

Original languageEnglish
Pages (from-to)1217-1223
Number of pages7
JournalOpen Biology
Volume3
Issue number12
Early online date21 Nov 2014
DOIs
Publication statusPublished - 15 Dec 2014

    Fingerprint

Keywords

  • Clasp
  • K-fiber
  • Microtubules
  • Mitosis
  • Spindle

Cite this