Leptin activates ATP-sensitive potassium channels in the rat insulin-secreting cell line, CRI-G1

J Harvey, F McKenna, P S Herson, D Spanswick, M L Ashford

    Research output: Contribution to journalArticle

    89 Citations (Scopus)

    Abstract

    1. Whole-cell current-clamp recordings demonstrate that leptin (0.3-10 nm) hyperpolarizes CRI-G1 insulin-secreting cells. This effect is slow on onset and is not reversed on washout of the leptin. 2. Voltage-clamp recordings indicate that leptin activates a potassium conductance in the presence of intracellular ATP (5 mm), but has not effect in its absence. Following activation of ATP-sensitive K+ (KATP) current by diazoxide (0.2 mm), addition of leptin did not alter cell membrane potential or potassium current further. 3. The leptin-induced hyperpolarization and increased potassium conductance are completely inhibited by the application of the sulphonylureas tolbutamide (100 microM) and glibenclamide (0.5 microM). 4. Cell-attached and inside-out single-channel recordings indicate that leptin activates tolbutamide-sensitive KATP channels in CRI-G1 insulin-secreting cells.
    Original languageEnglish
    Pages (from-to)527-35
    Number of pages9
    JournalJournal of Physiology
    Volume504
    Issue number( Pt 3)
    Publication statusPublished - 1 Nov 1997

    Keywords

    • Animals
    • Ion Channel Gating
    • Electrophysiology
    • Insulin
    • Potassium Channels
    • Rats
    • Leptin
    • Hypoglycemic Agents
    • Patch-Clamp Techniques
    • Biotransformation
    • Membrane Potentials
    • Proteins
    • Tolbutamide
    • Adenosine Triphosphate
    • Cell Line

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