Light-sheet microscopy using an Airy beam

Tom Vettenburg; Heather I. C. Dalgarno; Jonathan Nylk; Clara Coll-Lladó; David E. K. Ferrier; Tomáš Čižmár; Frank J. Gunn-Moore; Kishan Dholakia

doi:10.1038/nmeth.2922

Light-sheet microscopy using an Airy beam

Tom Vettenburg (Lead / Corresponding author), Heather I. C. Dalgarno, Jonathan Nylk, Clara Coll-Lladó, David E. K. Ferrier, Tomáš Čižmár, Frank J. Gunn-Moore, Kishan Dholakia (Lead / Corresponding author)

Physics

Research output: Contribution to journal › Article › peer-review

730 Citations (Scopus)

Abstract

Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

Original language	English
Pages (from-to)	541-544
Number of pages	4
Journal	Nature Methods
Volume	11
Issue number	5
Early online date	6 Apr 2014
DOIs	https://doi.org/10.1038/nmeth.2922
Publication status	Published - May 2014

Keywords

Cellular imaging
Fluorescence imaging
Light-sheet microscopy

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Biochemistry
Cell Biology

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10.1038/nmeth.2922

Cite this

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title = "Light-sheet microscopy using an Airy beam",

abstract = "Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.",

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AU - Vettenburg, Tom

AU - Dalgarno, Heather I. C.

AU - Nylk, Jonathan

AU - Coll-Lladó, Clara

AU - Ferrier, David E. K.

AU - Čižmár, Tomáš

AU - Gunn-Moore, Frank J.

AU - Dholakia, Kishan

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AB - Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

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KW - Fluorescence imaging

KW - Light-sheet microscopy

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Light-sheet microscopy using an Airy beam

Abstract

Keywords

ASJC Scopus subject areas

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