Light-sheet microscopy with attenuation-compensated propagation-invariant beams

TY - JOUR

T1 - Light-sheet microscopy with attenuation-compensated propagation-invariant beams

AU - Nylk, Jonathan

AU - McCluskey, Kaley

AU - Preciado, Miguel A.

AU - Mazilu, Michael

AU - Yang, Zhengyi

AU - Gunn-Moore, Frank J.

AU - Aggarwal, Sanya

AU - Tello, Javier A.

AU - Ferrier, David E. K.

AU - Dholakia, Kishan

PY - 2018/4/6

Y1 - 2018/4/6

N2 - Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.

AB - Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.

UR - https://www.scopus.com/pages/publications/85045022515

U2 - 10.1126/sciadv.aar4817

DO - 10.1126/sciadv.aar4817

M3 - Article

C2 - 29740614

AN - SCOPUS:85045022515

SN - 2375-2548

VL - 4

JO - Science Advances

JF - Science Advances

IS - 4

M1 - eaar4817

ER -