LINE-1 methylation in cleft lip tissues

influence of infant MTHFR c.677C>T genotype

Faisel Khan, Julian Little, Valentina Aleotti, Peter Mossey, Régine P. M. Steegers-Theunissen, Luca Autelitano, Maria Costanza Meazzini, Amin Ravaei, Michele Rubini (Lead / Corresponding author)

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the influence of MTHFR c.677C>T genotype on LINE-1 methylation in lateral and medial tissues from cleft lip (CL). Methods: Forty-five consecutive non-syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE-1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. Results: LINE-1 methylation level was significantly higher on medial side of cleft lip compared with lateral side (p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE-1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases (p = 0.027). Conclusion: This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.

Original languageEnglish
Number of pages4
JournalOral Diseases
Early online date4 Jun 2019
DOIs
Publication statusE-pub ahead of print - 4 Jun 2019

Fingerprint

Cleft Lip
Methylation
Genotype
Cleft Palate
DNA Methylation
Alleles
DNA

Keywords

  • DNA methylation
  • LINE-1
  • MTHFR c.677C>T
  • non-syndromic cleft lip with or without cleft palate

Cite this

Khan, F., Little, J., Aleotti, V., Mossey, P., Steegers-Theunissen, R. P. M., Autelitano, L., ... Rubini, M. (2019). LINE-1 methylation in cleft lip tissues: influence of infant MTHFR c.677C>T genotype. Oral Diseases. https://doi.org/10.1111/odi.13136
Khan, Faisel ; Little, Julian ; Aleotti, Valentina ; Mossey, Peter ; Steegers-Theunissen, Régine P. M. ; Autelitano, Luca ; Meazzini, Maria Costanza ; Ravaei, Amin ; Rubini, Michele. / LINE-1 methylation in cleft lip tissues : influence of infant MTHFR c.677C>T genotype. In: Oral Diseases. 2019.
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abstract = "Objective: To investigate the influence of MTHFR c.677C>T genotype on LINE-1 methylation in lateral and medial tissues from cleft lip (CL). Methods: Forty-five consecutive non-syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE-1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. Results: LINE-1 methylation level was significantly higher on medial side of cleft lip compared with lateral side (p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE-1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases (p = 0.027). Conclusion: This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.",
keywords = "DNA methylation, LINE-1, MTHFR c.677C>T, non-syndromic cleft lip with or without cleft palate",
author = "Faisel Khan and Julian Little and Valentina Aleotti and Peter Mossey and Steegers-Theunissen, {R{\'e}gine P. M.} and Luca Autelitano and Meazzini, {Maria Costanza} and Amin Ravaei and Michele Rubini",
note = "This study was partly supported by FAR-2018 grant from University of Ferrara and from Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Italy, Grant/Award Number: 62040 and 0039804",
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Khan, F, Little, J, Aleotti, V, Mossey, P, Steegers-Theunissen, RPM, Autelitano, L, Meazzini, MC, Ravaei, A & Rubini, M 2019, 'LINE-1 methylation in cleft lip tissues: influence of infant MTHFR c.677C>T genotype', Oral Diseases. https://doi.org/10.1111/odi.13136

LINE-1 methylation in cleft lip tissues : influence of infant MTHFR c.677C>T genotype. / Khan, Faisel; Little, Julian ; Aleotti, Valentina ; Mossey, Peter; Steegers-Theunissen, Régine P. M.; Autelitano, Luca; Meazzini, Maria Costanza; Ravaei, Amin ; Rubini, Michele (Lead / Corresponding author).

In: Oral Diseases, 04.06.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - LINE-1 methylation in cleft lip tissues

T2 - influence of infant MTHFR c.677C>T genotype

AU - Khan, Faisel

AU - Little, Julian

AU - Aleotti, Valentina

AU - Mossey, Peter

AU - Steegers-Theunissen, Régine P. M.

AU - Autelitano, Luca

AU - Meazzini, Maria Costanza

AU - Ravaei, Amin

AU - Rubini, Michele

N1 - This study was partly supported by FAR-2018 grant from University of Ferrara and from Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Italy, Grant/Award Number: 62040 and 0039804

PY - 2019/6/4

Y1 - 2019/6/4

N2 - Objective: To investigate the influence of MTHFR c.677C>T genotype on LINE-1 methylation in lateral and medial tissues from cleft lip (CL). Methods: Forty-five consecutive non-syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE-1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. Results: LINE-1 methylation level was significantly higher on medial side of cleft lip compared with lateral side (p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE-1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases (p = 0.027). Conclusion: This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.

AB - Objective: To investigate the influence of MTHFR c.677C>T genotype on LINE-1 methylation in lateral and medial tissues from cleft lip (CL). Methods: Forty-five consecutive non-syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE-1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. Results: LINE-1 methylation level was significantly higher on medial side of cleft lip compared with lateral side (p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE-1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases (p = 0.027). Conclusion: This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.

KW - DNA methylation

KW - LINE-1

KW - MTHFR c.677C>T

KW - non-syndromic cleft lip with or without cleft palate

UR - http://www.scopus.com/inward/record.url?scp=85067847942&partnerID=8YFLogxK

U2 - 10.1111/odi.13136

DO - 10.1111/odi.13136

M3 - Article

JO - Oral Diseases

JF - Oral Diseases

SN - 1354-523X

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