Projects per year
Abstract
The vertebrate body forms by continuous generation of new tissue from progenitors at the posterior end of the embryo. The study of these axial progenitors has proved challenging in vivo largely due to the lack of unique molecular markers to identify them. Here, we elucidate the expression pattern of the transcription factor Nkx1-2 in the mouse embryo and show that it identifies axial progenitors throughout body axis elongation, including neuromesodermal progenitors and early neural and mesodermal progenitors. We create a tamoxifen-inducible Nkx1-2CreERT2 37 transgenic mouse and exploit the conditional nature of this line to uncover the lineage contributions of Nkx1-2-expressing cells at specific stages. We show that early Nkx1-2-expressing epiblast cells contribute to all three germ layers, mostly neuroectoderm and mesoderm, excluding notochord. Our data are consistent with the presence of some self-renewing axial progenitors that continue to generate neural and mesoderm tissues from the tail bud. This study identifies Nkx1-2 expressing cells as the source of most trunk and tail tissues in the mouse and provides a useful tool to genetically label and manipulate axial progenitors in vivo.
Original language | English |
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Article number | 164319 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Development |
Volume | 145 |
Issue number | 19 |
DOIs | |
Publication status | Published - 10 Sept 2018 |
Keywords
- axial progenitors
- neuromesodermal progenitors
- Nkx1-2
- body axis elongation
- genetic lineage tracing
- mouse embryo
- Neuromesodermal progenitors
- Genetic lineage tracing
- Mouse embryo
- Axial progenitors
- Body axis elongation
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
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Dive into the research topics of 'Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions'. Together they form a unique fingerprint.Projects
- 2 Finished
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Cellular and Molecular Mechanisms Regulating Neuronal Differentiation in Embryos and Adults (Senior Investigator award)
Storey, K. (Investigator)
21/07/14 → 31/12/21
Project: Research
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Development of Live Tissue Imaging Capabilities Using Multiphoton Confocal Microscopy
Muller, A. (Investigator), Nathke, I. (Investigator), Storey, K. (Investigator) & Weijer, K. (Investigator)
30/06/14 → 29/06/19
Project: Research