Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade

Guang Wang, Pei-Zhi Li, Shi-Yao Zhang, Shan Zhong, Chang Chu, Shufei Zeng, Yu Yan, Xin Cheng, Manli Chuai, Berthold Hocher (Lead / Corresponding author), Xuesong Yang (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)
    178 Downloads (Pure)


    Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far.

    Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR.

    Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade.

    Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade.

    Original languageEnglish
    Pages (from-to)2084-2090
    Number of pages7
    JournalCellular Physiology and Biochemistry
    Issue number5
    Early online date10 Aug 2018
    Publication statusPublished - Aug 2018


    • Angiogenesis
    • Chicken chorioallantoic membrane (CAM)
    • Endothelin (ET)
    • Lipopolysaccharides (LPS)

    ASJC Scopus subject areas

    • Physiology


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