Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade

Guang Wang, Pei-Zhi Li, Shi-Yao Zhang, Shan Zhong, Chang Chu, Shufei Zeng, Yu Yan, Xin Cheng, Manli Chuai, Berthold Hocher (Lead / Corresponding author), Xuesong Yang (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far.

Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR.

Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade.

Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade.

Original languageEnglish
Pages (from-to)2084-2090
Number of pages7
JournalCellular Physiology and Biochemistry
Volume48
Issue number5
Early online date10 Aug 2018
DOIs
Publication statusPublished - Aug 2018

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Embryonic Development
Lipopolysaccharides
Chickens
Endothelins
Endothelin A Receptors
Blood Vessels
Endothelin-2
Endothelin B Receptors
Chorioallantoic Membrane
Endothelin-1
Gene Expression
Polymerase Chain Reaction
Messenger RNA
cyclo(Trp-Asp-Pro-Val-Leu)
BQ 788

Keywords

  • Angiogenesis
  • Chicken chorioallantoic membrane (CAM)
  • Endothelin (ET)
  • Lipopolysaccharides (LPS)

Cite this

Wang, Guang ; Li, Pei-Zhi ; Zhang, Shi-Yao ; Zhong, Shan ; Chu, Chang ; Zeng, Shufei ; Yan, Yu ; Cheng, Xin ; Chuai, Manli ; Hocher, Berthold ; Yang, Xuesong. / Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade. In: Cellular Physiology and Biochemistry. 2018 ; Vol. 48, No. 5. pp. 2084-2090.
@article{ce2b6ecf6613440a8af15862aa49e3dc,
title = "Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade",
abstract = "Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far.Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR.Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade.Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade.",
keywords = "Angiogenesis, Chicken chorioallantoic membrane (CAM), Endothelin (ET), Lipopolysaccharides (LPS)",
author = "Guang Wang and Pei-Zhi Li and Shi-Yao Zhang and Shan Zhong and Chang Chu and Shufei Zeng and Yu Yan and Xin Cheng and Manli Chuai and Berthold Hocher and Xuesong Yang",
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year = "2018",
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Wang, G, Li, P-Z, Zhang, S-Y, Zhong, S, Chu, C, Zeng, S, Yan, Y, Cheng, X, Chuai, M, Hocher, B & Yang, X 2018, 'Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade', Cellular Physiology and Biochemistry, vol. 48, no. 5, pp. 2084-2090. https://doi.org/10.1159/000492547

Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade. / Wang, Guang; Li, Pei-Zhi; Zhang, Shi-Yao; Zhong, Shan; Chu, Chang; Zeng, Shufei; Yan, Yu; Cheng, Xin; Chuai, Manli; Hocher, Berthold (Lead / Corresponding author); Yang, Xuesong (Lead / Corresponding author).

In: Cellular Physiology and Biochemistry, Vol. 48, No. 5, 08.2018, p. 2084-2090.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade

AU - Wang, Guang

AU - Li, Pei-Zhi

AU - Zhang, Shi-Yao

AU - Zhong, Shan

AU - Chu, Chang

AU - Zeng, Shufei

AU - Yan, Yu

AU - Cheng, Xin

AU - Chuai, Manli

AU - Hocher, Berthold

AU - Yang, Xuesong

N1 - © 2018 The Author(s). Published by S. Karger AG, Basel.

PY - 2018/8

Y1 - 2018/8

N2 - Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far.Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR.Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade.Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade.

AB - Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far.Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR.Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade.Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade.

KW - Angiogenesis

KW - Chicken chorioallantoic membrane (CAM)

KW - Endothelin (ET)

KW - Lipopolysaccharides (LPS)

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DO - 10.1159/000492547

M3 - Article

C2 - 30099448

VL - 48

SP - 2084

EP - 2090

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

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