Live cell imaging using wide-field microscopy and deconvolution

Jason R. Swedlow (Lead / Corresponding author), Melpomeni Platani

    Research output: Contribution to journalReview articlepeer-review

    79 Citations (Scopus)


    The use of fluorescence imaging methods, most recently based on fluorescent protein technology, and the availability of high quality fluorescence imaging systems have driven a revolution in cell and molecular biology. Live cell imaging, especially using fluorescence, is now used in a wide variety of assays in academic and commercial laboratories. The use of this technology requires particular attention to be paid to cell engineering, the design of the image acquisition system, the imaging protocol, and subsequent processing and analytic methods. In this review, we discuss each of these steps, highlighting practical techniques developed by us and others.

    Original languageEnglish
    Pages (from-to)335-341
    Number of pages7
    JournalCell Structure and Function
    Issue number5
    Publication statusPublished - Oct 2002


    • Deconvolution
    • Fluorescence
    • GFP
    • Live cell imaging
    • Microscopy

    ASJC Scopus subject areas

    • Physiology
    • Molecular Biology
    • Cell Biology


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