Live cell imaging using wide-field microscopy and deconvolution

TY - JOUR

T1 - Live cell imaging using wide-field microscopy and deconvolution

AU - Swedlow, Jason R.

AU - Platani, Melpomeni

PY - 2002/10

Y1 - 2002/10

N2 - The use of fluorescence imaging methods, most recently based on fluorescent protein technology, and the availability of high quality fluorescence imaging systems have driven a revolution in cell and molecular biology. Live cell imaging, especially using fluorescence, is now used in a wide variety of assays in academic and commercial laboratories. The use of this technology requires particular attention to be paid to cell engineering, the design of the image acquisition system, the imaging protocol, and subsequent processing and analytic methods. In this review, we discuss each of these steps, highlighting practical techniques developed by us and others.

AB - The use of fluorescence imaging methods, most recently based on fluorescent protein technology, and the availability of high quality fluorescence imaging systems have driven a revolution in cell and molecular biology. Live cell imaging, especially using fluorescence, is now used in a wide variety of assays in academic and commercial laboratories. The use of this technology requires particular attention to be paid to cell engineering, the design of the image acquisition system, the imaging protocol, and subsequent processing and analytic methods. In this review, we discuss each of these steps, highlighting practical techniques developed by us and others.

KW - Deconvolution

KW - Fluorescence

KW - GFP

KW - Live cell imaging

KW - Microscopy

UR - https://www.scopus.com/pages/publications/0036823318

U2 - 10.1247/csf.27.335

DO - 10.1247/csf.27.335

M3 - Review article

C2 - 12502887

AN - SCOPUS:0036823318

SN - 0386-7196

VL - 27

SP - 335

EP - 341

JO - Cell Structure and Function

JF - Cell Structure and Function

IS - 5

ER -