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When human cells enter mitosis, chromosomes undergo substantial changes in their organization to resolve sister chromatids and compact chromosomes. To comprehend the timing and coordination of these events, we need to evaluate the progression of both sister chromatid resolution and chromosome compaction in one assay. Here we achieved this by analyzing changes in configuration of marked chromosome regions over time, with high spatial and temporal resolution. This assay showed that sister chromatids cycle between nonresolved and partially resolved states with an interval of a few minutes during G2 phase before completing full resolution in prophase. Cohesins and WAPL antagonistically regulate sister chromatid resolution in late G2 and prophase while local enrichment of cohesin on chromosomes prevents precocious sister chromatid resolution. Moreover, our assay allowed quantitative evaluation of condensin II and I activities, which differentially promote sister chromatid resolution and chromosome compaction, respectively. Our assay reveals novel aspects of dynamics in mitotic chromosome resolution and compaction that were previously obscure in global chromosome assays.
FingerprintDive into the research topics of 'Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis'. Together they form a unique fingerprint.
- 4 Finished
1/04/13 → 30/09/18
1/01/13 → 30/09/18
- 10 Citations
- 1 Preprint
Live-cell imaging of marked chromosome regions reveals dynamics of mitotic chromosome resolution and compactionEykelenboom, J. K., Gierlinski, M., Yue, Z., Hegarat, N., Pollard, H., Fukagawa, T., Hochegger, H. & Tanaka, T. U., 17 May 2018, Cold Spring Harbor Laboratory: BioRxiv, 53 p.
Research output: Working paper/Preprint › PreprintFile116 Downloads (Pure)