Live TIRF fluorescence imaging at 100nm resolution through structured illumination

P. Kner, B. Chhun, E. Griffis, L. Winoto, L. Shao, M. G. L. Gustafsson

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.

    Original languageEnglish
    Title of host publicationThree-Dimensional and Multidimensional Microscopy
    Subtitle of host publicationImage Acquisition and Processing XVI
    EditorsJose-Angel Conchello, Carol J. Cogswell, Tony Wilson
    Place of PublicationBellingham
    PublisherSPIE-International Society for Optical Engineering
    Number of pages9
    ISBN (Print)9780819474308
    DOIs
    Publication statusPublished - 2009
    EventSPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI - Marriott Hotel, San Jose Ballroom Salon III, San Jose, United States
    Duration: 26 Jan 200929 Jan 2009
    http://spie.org/x33616.xml

    Publication series

    NameProceedings of SPIE
    PublisherSPIE
    Volume7184

    Conference

    ConferenceSPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
    CountryUnited States
    CitySan Jose
    Period26/01/0929/01/09
    Internet address

    Keywords

    • Structured illumination
    • Super-resolution
    • Microscopy
    • Bio-imaging
    • LOCALIZATION MICROSCOPY
    • DYNAMICS
    • SPINDLE
    • PROTEIN
    • CELLS
    • LIMIT

    Cite this

    Kner, P., Chhun, B., Griffis, E., Winoto, L., Shao, L., & Gustafsson, M. G. L. (2009). Live TIRF fluorescence imaging at 100nm resolution through structured illumination. In J-A. Conchello, C. J. Cogswell, & T. Wilson (Eds.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI [718417] (Proceedings of SPIE; Vol. 7184). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.812351