Live TIRF fluorescence imaging at 100nm resolution through structured illumination

P. Kner; B. Chhun; E. Griffis; L. Winoto; L. Shao; M. G. L. Gustafsson

doi:10.1117/12.812351

Live TIRF fluorescence imaging at 100nm resolution through structured illumination

P. Kner, B. Chhun, E. Griffis, L. Winoto, L. Shao, M. G. L. Gustafsson

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.

Original language	English
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XVI
Editors	Jose-Angel Conchello, Carol J. Cogswell, Tony Wilson
Place of Publication	Bellingham
Publisher	SPIE-International Society for Optical Engineering
Number of pages	9
ISBN (Print)	9780819474308
DOIs	https://doi.org/10.1117/12.812351
Publication status	Published - 2009
Event	SPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI - Marriott Hotel, San Jose Ballroom Salon III, San Jose, United States Duration: 26 Jan 2009 → 29 Jan 2009 http://spie.org/x33616.xml

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	7184

Conference

Conference	SPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
Country/Territory	United States
City	San Jose
Period	26/01/09 → 29/01/09
Internet address	http://spie.org/x33616.xml

Keywords

Structured illumination
Super-resolution
Microscopy
Bio-imaging
LOCALIZATION MICROSCOPY
DYNAMICS
SPINDLE
PROTEIN
CELLS
LIMIT

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10.1117/12.812351

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Kner, P., Chhun, B., Griffis, E., Winoto, L., Shao, L., & Gustafsson, M. G. L. (2009). Live TIRF fluorescence imaging at 100nm resolution through structured illumination. In J.-A. Conchello, C. J. Cogswell, & T. Wilson (Eds.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI Article 718417 (Proceedings of SPIE; Vol. 7184). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.812351

Kner, P. ; Chhun, B. ; Griffis, E. et al. / Live TIRF fluorescence imaging at 100nm resolution through structured illumination. Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI. editor / Jose-Angel Conchello ; Carol J. Cogswell ; Tony Wilson. Bellingham : SPIE-International Society for Optical Engineering, 2009. (Proceedings of SPIE).

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title = "Live TIRF fluorescence imaging at 100nm resolution through structured illumination",

abstract = "Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.",

keywords = "Structured illumination, Super-resolution, Microscopy, Bio-imaging, LOCALIZATION MICROSCOPY, DYNAMICS, SPINDLE, PROTEIN, CELLS, LIMIT",

author = "P. Kner and B. Chhun and E. Griffis and L. Winoto and L. Shao and Gustafsson, {M. G. L.}",

year = "2009",

doi = "10.1117/12.812351",

language = "English",

isbn = "9780819474308",

series = "Proceedings of SPIE",

publisher = "SPIE-International Society for Optical Engineering",

editor = "Jose-Angel Conchello and Cogswell, {Carol J.} and Tony Wilson",

booktitle = "Three-Dimensional and Multidimensional Microscopy",

note = "SPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI ; Conference date: 26-01-2009 Through 29-01-2009",

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Kner, P, Chhun, B, Griffis, E, Winoto, L, Shao, L & Gustafsson, MGL 2009, Live TIRF fluorescence imaging at 100nm resolution through structured illumination. in J-A Conchello, CJ Cogswell & T Wilson (eds), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI., 718417, Proceedings of SPIE, vol. 7184, SPIE-International Society for Optical Engineering, Bellingham, SPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, San Jose, United States, 26/01/09. https://doi.org/10.1117/12.812351

Live TIRF fluorescence imaging at 100nm resolution through structured illumination. / Kner, P.; Chhun, B.; Griffis, E. et al.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI. ed. / Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson. Bellingham: SPIE-International Society for Optical Engineering, 2009. 718417 (Proceedings of SPIE; Vol. 7184).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Live TIRF fluorescence imaging at 100nm resolution through structured illumination

AU - Kner, P.

AU - Chhun, B.

AU - Griffis, E.

AU - Winoto, L.

AU - Shao, L.

AU - Gustafsson, M. G. L.

PY - 2009

Y1 - 2009

N2 - Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.

AB - Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.

KW - Structured illumination

KW - Super-resolution

KW - Microscopy

KW - Bio-imaging

KW - LOCALIZATION MICROSCOPY

KW - DYNAMICS

KW - SPINDLE

KW - PROTEIN

KW - CELLS

KW - LIMIT

U2 - 10.1117/12.812351

DO - 10.1117/12.812351

M3 - Conference contribution

SN - 9780819474308

T3 - Proceedings of SPIE

BT - Three-Dimensional and Multidimensional Microscopy

A2 - Conchello, Jose-Angel

A2 - Cogswell, Carol J.

A2 - Wilson, Tony

PB - SPIE-International Society for Optical Engineering

CY - Bellingham

T2 - SPIE Photonics West 2009: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI

Y2 - 26 January 2009 through 29 January 2009

ER -

Kner P, Chhun B, Griffis E, Winoto L, Shao L, Gustafsson MGL. Live TIRF fluorescence imaging at 100nm resolution through structured illumination. In Conchello JA, Cogswell CJ, Wilson T, editors, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI. Bellingham: SPIE-International Society for Optical Engineering. 2009. 718417. (Proceedings of SPIE). doi: 10.1117/12.812351

Live TIRF fluorescence imaging at 100nm resolution through structured illumination

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