Local anesthetics induce autophagy in young permanent tooth pulp cells

H. Zhuang, D. Hu, D. Singer, J. V. Walker, R. B. Nisr, K. Tieu, K. Ali, C. Tredwin, S. Luo, S. Ardu, B. Hu (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)
    15 Downloads (Pure)

    Abstract

    Pulp cells are essential for tooth development, and dentin repair and regeneration. In addition these cells have been identified as an important stem cell source. Local anesthetics are widely used in dental clinics, as well as the other clinical disciplines and have been suggested to interfere with human permanent tooth development and induce tooth agenesis through unknown mechanisms. Using pig model and human young permanent tooth pulp cells, our research has identified that the local anesthetics commonly used in clinics can affect cell proliferation. Molecular pathway profiling suggested that LC3II is one of the earliest molecules induced by the agents and p62 is the only common downstream target identified for all the drugs tested. The effect of the drugs could be partially recovered by V-ATPase inhibitor only if early intervention is performed. Our results provide novel evidence that local anesthetics could affect tooth cell growth that potentially can have impacts on tooth development.

    Original languageEnglish
    Article number15024
    Number of pages9
    JournalCell Death Discovery
    Volume1
    Issue number1
    Early online date7 Sept 2015
    DOIs
    Publication statusPublished - 21 Dec 2015

    Keywords

    • Mitophagy
    • TOR signalling

    ASJC Scopus subject areas

    • Immunology
    • Cellular and Molecular Neuroscience
    • Cell Biology
    • Cancer Research

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