Localisation of alpha, mu and pi class glutathione S-transferases in kidney

comparison with CuZn superoxide dismutase

Simon J. Davies, Richard D'Sousa, Helen Philips, Derek Mattey, Christopher Hiley, John D. Hayes, Geoffrey M. Aber, Richard C. Strange

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We describe studies in whole kidney, cortical and medullary homogenates and, glomerular cells in culture to determine the relative levels of expression of alpha (Ya, Yc, Yk), mu (Yb1/Yb2), pi (Yf) glutathione S-transferases (GST) and CuZn superoxide dismutase (CuZn SOD) in different regions of the nephron. Immunoblotting and immunohistochemistry were used to demonstrate relatively weak expression of alpha, mu GST and, CuZn SOD in the glomerulus compared to that in particularly distal tubules. Whilst expression of Ya was found within glomerular cells, Yc, Yk and Yf were not detected. Immunofluorescence showed that Ya and Yb1/Yb2 but not Yf were expressed in cultured epithelial and mesangial cells studied between passages 1 and 3. While Ya was distributed in cytosol, Yb1/Yb2 was primarily located in nuclei.

Original languageEnglish
Pages (from-to)204-8
Number of pages5
JournalBiochimica et Biophysica Acta: General Subjects
Volume1157
Issue number2
DOIs
Publication statusPublished - 11 Jun 1993

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Glutathione S-Transferase pi
Glutathione Transferase
Superoxide Dismutase
Kidney
Mesangial Cells
Nephrons
Immunoblotting
Cytosol
Fluorescent Antibody Technique
Cultured Cells
Cell Culture Techniques
Epithelial Cells
Immunohistochemistry
glutathione S-transferase alpha

Keywords

  • Animals
  • Cells, Cultured/enzymology
  • Fluorescent Antibody Technique
  • Glutathione Transferase/analysis
  • Immunoblotting
  • Kidney/enzymology
  • Kidney Cortex/enzymology
  • Kidney Medulla/enzymology
  • Nephrons/enzymology
  • Rats
  • Rats, Inbred WKY
  • Superoxide Dismutase/analysis

Cite this

Davies, Simon J. ; D'Sousa, Richard ; Philips, Helen ; Mattey, Derek ; Hiley, Christopher ; Hayes, John D. ; Aber, Geoffrey M. ; Strange, Richard C. / Localisation of alpha, mu and pi class glutathione S-transferases in kidney : comparison with CuZn superoxide dismutase. In: Biochimica et Biophysica Acta: General Subjects. 1993 ; Vol. 1157, No. 2. pp. 204-8.
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abstract = "We describe studies in whole kidney, cortical and medullary homogenates and, glomerular cells in culture to determine the relative levels of expression of alpha (Ya, Yc, Yk), mu (Yb1/Yb2), pi (Yf) glutathione S-transferases (GST) and CuZn superoxide dismutase (CuZn SOD) in different regions of the nephron. Immunoblotting and immunohistochemistry were used to demonstrate relatively weak expression of alpha, mu GST and, CuZn SOD in the glomerulus compared to that in particularly distal tubules. Whilst expression of Ya was found within glomerular cells, Yc, Yk and Yf were not detected. Immunofluorescence showed that Ya and Yb1/Yb2 but not Yf were expressed in cultured epithelial and mesangial cells studied between passages 1 and 3. While Ya was distributed in cytosol, Yb1/Yb2 was primarily located in nuclei.",
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Localisation of alpha, mu and pi class glutathione S-transferases in kidney : comparison with CuZn superoxide dismutase. / Davies, Simon J.; D'Sousa, Richard; Philips, Helen; Mattey, Derek; Hiley, Christopher; Hayes, John D.; Aber, Geoffrey M.; Strange, Richard C.

In: Biochimica et Biophysica Acta: General Subjects, Vol. 1157, No. 2, 11.06.1993, p. 204-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Localisation of alpha, mu and pi class glutathione S-transferases in kidney

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AU - Davies, Simon J.

AU - D'Sousa, Richard

AU - Philips, Helen

AU - Mattey, Derek

AU - Hiley, Christopher

AU - Hayes, John D.

AU - Aber, Geoffrey M.

AU - Strange, Richard C.

PY - 1993/6/11

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N2 - We describe studies in whole kidney, cortical and medullary homogenates and, glomerular cells in culture to determine the relative levels of expression of alpha (Ya, Yc, Yk), mu (Yb1/Yb2), pi (Yf) glutathione S-transferases (GST) and CuZn superoxide dismutase (CuZn SOD) in different regions of the nephron. Immunoblotting and immunohistochemistry were used to demonstrate relatively weak expression of alpha, mu GST and, CuZn SOD in the glomerulus compared to that in particularly distal tubules. Whilst expression of Ya was found within glomerular cells, Yc, Yk and Yf were not detected. Immunofluorescence showed that Ya and Yb1/Yb2 but not Yf were expressed in cultured epithelial and mesangial cells studied between passages 1 and 3. While Ya was distributed in cytosol, Yb1/Yb2 was primarily located in nuclei.

AB - We describe studies in whole kidney, cortical and medullary homogenates and, glomerular cells in culture to determine the relative levels of expression of alpha (Ya, Yc, Yk), mu (Yb1/Yb2), pi (Yf) glutathione S-transferases (GST) and CuZn superoxide dismutase (CuZn SOD) in different regions of the nephron. Immunoblotting and immunohistochemistry were used to demonstrate relatively weak expression of alpha, mu GST and, CuZn SOD in the glomerulus compared to that in particularly distal tubules. Whilst expression of Ya was found within glomerular cells, Yc, Yk and Yf were not detected. Immunofluorescence showed that Ya and Yb1/Yb2 but not Yf were expressed in cultured epithelial and mesangial cells studied between passages 1 and 3. While Ya was distributed in cytosol, Yb1/Yb2 was primarily located in nuclei.

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KW - Kidney Medulla/enzymology

KW - Nephrons/enzymology

KW - Rats

KW - Rats, Inbred WKY

KW - Superoxide Dismutase/analysis

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