Localization and domain characterization of Arabidopsis golgin candidates

Maita Latijnhouwers, Trudi Gillespie, Petra Boevink, Verena Kriechbaumer, Chris Hawes, Claudine M. Carvalho

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    Golgins are large coiled-coil proteins that play a role in tethering of vesicles to Golgi membranes and in maintaining the overall structure of the Golgi apparatus. Six Arabidopsis proteins with the structural characteristics of golgins were isolated and shown to locate to Golgi stacks when fused to GFP. Two of these golgin candidates (GC1 and GC2) possess C-terminal transmembrane (TM) domains with similarity to the TM domain of human golgin-84. The C-termini of two others (GC3/GDAP1 and GC4) contain conserved GRAB and GA1 domains that are also found in yeast Rud3p and human GMAP210. GC5 shares similarity with yeast Sgm1p and human TMF and GC6 with yeast Uso1p and human p115. When fused to GFP, the C-terminal domains of AtCASP and GC1 to GC6 localized to the Golgi, showing that they contain Golgi localization motifs. The N-termini, on the other hand, label the cytosol or nucleus. Immuno-gold labelling and co-expression with the cis Golgi Q-SNARE Memb11 resulted in a more detailed picture of the sub-Golgi location of some of these putative golgins. Using two independent assays it is further demonstrated that the interaction between GC5, the TMF homologue, and the Rab6 homologues is conserved in plants.
    Original languageEnglish
    Pages (from-to)4373-4386
    Number of pages14
    JournalJournal of Experimental Botany
    Volume58
    Issue number15-16
    DOIs
    Publication statusPublished - Dec 2007

    Keywords

    • Amino Acid Sequence
    • Animals
    • Arabidopsis
    • Arabidopsis Proteins
    • Golgi Apparatus
    • Green Fluorescent Proteins
    • Immunohistochemistry
    • Membrane Proteins
    • Molecular Sequence Data
    • Protein Structure, Tertiary
    • Sequence Homology, Amino Acid
    • Two-Hybrid System Techniques
    • rab GTP-Binding Proteins

    Fingerprint Dive into the research topics of 'Localization and domain characterization of <em>Arabidopsis</em> golgin candidates'. Together they form a unique fingerprint.

  • Cite this

    Latijnhouwers, M., Gillespie, T., Boevink, P., Kriechbaumer, V., Hawes, C., & Carvalho, C. M. (2007). Localization and domain characterization of Arabidopsis golgin candidates. Journal of Experimental Botany, 58(15-16), 4373-4386. https://doi.org/10.1093/jxb/erm304