Localization of cytochrome P450 CYP2S1 expression in human tissues by in situ hybridization and immunohistochemistry

TY - JOUR

T1 - Localization of cytochrome P450 CYP2S1 expression in human tissues by in situ hybridization and immunohistochemistry

AU - Saarikoski, Sirkku T.

AU - Wikman, Harriet A.L.

AU - Smith, Gillian

AU - Wolff, C. Henrik J.

AU - Husgafvel-Pursiainen, Kirsti

N1 - © The Histochemical Society, Inc. This work was financially supported by the Academy of Finland (grant no. 52276) and the Work Environment Fund(grant no. 100389).

PY - 2005/5

Y1 - 2005/5

N2 - CYP2S1 is a recently discovered dioxin-inducible member of the cytochrome P450 superfamily. It has been shown to be involved in the metabolism of some aromatic hydrocarbons as well as retinoic acid, suggesting a role in biotransformation of both exogenous and endogenous compounds. In this study, we used mRNA in situ hybridization and immunohistochemistry to investigate the cellular localization of CYP2S1 in various human tissues using tissue microarrays. High expression levels were observed mainly in epithelial cell types, especially in the epithelia frequently exposed to xenobiotics. In the respiratory tract, the expression was strong in nasal cavity, bronchi, and bronchioli, whereas it was low in the alveolar lining cells. Similarly, CYP2S1 was highly expressed in the epithelial cells throughout the gastrointestinal tract. Strong epithelial expression was also observed in uterine cervix, urinary bladder, and skin. In many exocrine glands (e.g., adrenal gland and pancreas), secretory epithelial cells showed moderate to strong expression levels. In the liver, the expression was low. CYP2S1 was highly expressed in epithelial cells that are major targets for carcinogen exposure and common progenitor cells to tumor development. Indeed, we found strong CYP2S1 expression in many tumors of epithelial origin.

AB - CYP2S1 is a recently discovered dioxin-inducible member of the cytochrome P450 superfamily. It has been shown to be involved in the metabolism of some aromatic hydrocarbons as well as retinoic acid, suggesting a role in biotransformation of both exogenous and endogenous compounds. In this study, we used mRNA in situ hybridization and immunohistochemistry to investigate the cellular localization of CYP2S1 in various human tissues using tissue microarrays. High expression levels were observed mainly in epithelial cell types, especially in the epithelia frequently exposed to xenobiotics. In the respiratory tract, the expression was strong in nasal cavity, bronchi, and bronchioli, whereas it was low in the alveolar lining cells. Similarly, CYP2S1 was highly expressed in the epithelial cells throughout the gastrointestinal tract. Strong epithelial expression was also observed in uterine cervix, urinary bladder, and skin. In many exocrine glands (e.g., adrenal gland and pancreas), secretory epithelial cells showed moderate to strong expression levels. In the liver, the expression was low. CYP2S1 was highly expressed in epithelial cells that are major targets for carcinogen exposure and common progenitor cells to tumor development. Indeed, we found strong CYP2S1 expression in many tumors of epithelial origin.

KW - Cellular expression

KW - CYP2S1

KW - Cytochrome P450

KW - Human cancer

KW - Immunohistochemistry

KW - In situ hybridization

KW - Xenobiotic metabolism

UR - https://www.scopus.com/pages/publications/17844390622

U2 - 10.1369/jhc.4C6576.2005

DO - 10.1369/jhc.4C6576.2005

M3 - Article

C2 - 15872048

AN - SCOPUS:17844390622

SN - 0022-1554

VL - 53

SP - 549

EP - 556

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

IS - 5

ER -