Here, we show that transcription factors bound to regulatory sequences can be identified by purifying these unique sequences directly from mammalian cells invivo. Using targeted chromatin purification (TChP), a double-pull-down strategy with a tetracycline-sensitive "hook" bound to a specific promoter, we identify transcription factors bound to the repressed ?-globin gene-associated regulatory regions. After validation of the binding, we show that, in human primary erythroid cells, knockdown of a number of these transcription factors induces ?-globin gene expression. Reactivation of ?-globin gene expression ameliorates the symptoms of ß-thalassemia and sickle cell disease, and these factors provide potential targets for the development of therapeutics for treating these patients. •Purification of single-copy locus chromatin•Proteomic identification of protein factors bound to this chromatin•Recruitment of the identified factors to the ß-globin chromatin hub•Functional analysis of identified factors on ?-globin gene transcriptional regulation. Grosveld and colleagues show that proteins bound to unique sequences in the genome can be purified at levels sufficient for identification by mass spectrometry. Applying this technique, the authors identified a number of known factors as well as additional proteins that are potentially involved in the suppression of the human ?-globin genes.