TY - JOUR
T1 - Long-term within- and between-subject biological variation of 29 routine laboratory measurands in athletes
AU - Diaz-Garzon, Jorge
AU - Fernandez-Calle, Pilar
AU - Aarsand, Aasne K.
AU - Sandberg, Sverre
AU - Coskun, Abdurrahaman
AU - Carobene, Anna
AU - Jonker, Niels
AU - Itkonen, Outi
AU - Bartlett, William A.
AU - Buno, Antonio
AU - European Federation of Clinical Chemistry
N1 - © 2021 Walter de Gruyter GmbH, Berlin/Boston.
PY - 2022/3/4
Y1 - 2022/3/4
N2 - Objectives: Within- and between-subject biological variation (BV) estimates have many applications in laboratory medicine. However, robust high-quality BV estimates are lacking for many populations, such as athletes. This study aimed to deliver BV estimates of 29 routine laboratory measurands derived from a Biological Variation Data Critical Appraisal Checklist compliant design in a population of high-endurance athletes.Methods: Eleven samples per subject were drawn from 30 triathletes monthly, during a whole sport season. Serum samples were measured in duplicate for proteins, liver enzymes, lipids and kidney-related measurands on an Advia2400 (Siemens Healthineers). After outlier and homogeneity analysis, within-subject (CVI) and between-subject (CVG) biological variation estimates were delivered (CV-ANOVA and log-ANOVA, respectively) and a linear mixed model was applied to analyze the effect of exercise and health related variables.Results: Most CVI estimates were similar or only slightly higher in athletes compared to those reported for the general population, whereas two- to three-fold increases were observed for amylase, ALT, AST and ALP. No effect of exercise and health related variables were observed on the CVI estimates. For seven measurands, data were not homogeneously distributed and BV estimates were therefore not reported.Conclusions: The observation of higher CVI estimates in athletes than what has been reported for the general population may be related to physiological stress over time caused by the continuous practice of exercise. The BV estimates derived from this study could be applied to athlete populations from disciplines in which they exercise under similar conditions of intensity and duration.
AB - Objectives: Within- and between-subject biological variation (BV) estimates have many applications in laboratory medicine. However, robust high-quality BV estimates are lacking for many populations, such as athletes. This study aimed to deliver BV estimates of 29 routine laboratory measurands derived from a Biological Variation Data Critical Appraisal Checklist compliant design in a population of high-endurance athletes.Methods: Eleven samples per subject were drawn from 30 triathletes monthly, during a whole sport season. Serum samples were measured in duplicate for proteins, liver enzymes, lipids and kidney-related measurands on an Advia2400 (Siemens Healthineers). After outlier and homogeneity analysis, within-subject (CVI) and between-subject (CVG) biological variation estimates were delivered (CV-ANOVA and log-ANOVA, respectively) and a linear mixed model was applied to analyze the effect of exercise and health related variables.Results: Most CVI estimates were similar or only slightly higher in athletes compared to those reported for the general population, whereas two- to three-fold increases were observed for amylase, ALT, AST and ALP. No effect of exercise and health related variables were observed on the CVI estimates. For seven measurands, data were not homogeneously distributed and BV estimates were therefore not reported.Conclusions: The observation of higher CVI estimates in athletes than what has been reported for the general population may be related to physiological stress over time caused by the continuous practice of exercise. The BV estimates derived from this study could be applied to athlete populations from disciplines in which they exercise under similar conditions of intensity and duration.
KW - athletes
KW - biological variation
KW - endurance exercise
KW - long-term period
KW - routine laboratory measurands
UR - http://www.scopus.com/inward/record.url?scp=85120091096&partnerID=8YFLogxK
U2 - 10.1515/cclm-2021-0910
DO - 10.1515/cclm-2021-0910
M3 - Article
C2 - 34800014
SN - 1434-6621
VL - 60
SP - 618
EP - 628
JO - Clinical Chemistry and Laboratory Medicine (CCLM)
JF - Clinical Chemistry and Laboratory Medicine (CCLM)
IS - 4
ER -