Lost in translation: pitfalls in deciphering plant alternative splicing transcripts

John W. S. Brown (Lead / Corresponding author), Craig G. Simpson, Yamile Marquez, Geoffrey M. Gadd, Andrea Barta, Maria Kalyna

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Transcript annotation in plant databases is incomplete and often inaccurate, leading to misinterpretation. As more and more RNA-seq data are generated, plant scientists need to be aware of potential pitfalls and understand the nature and impact of specific alternative splicing transcripts on protein production. A primary area of concern and the topic of this article is the (mis)annotation of open reading frames and premature termination codons. The basic message is that to adequately address expression and functions of transcript isoforms, it is necessary to be able to predict their fate in terms of whether protein isoforms are generated or specific transcripts are unproductive or degraded.

Original languageEnglish
Pages (from-to)2083-2087
Number of pages5
JournalPlant Cell
Volume27
Issue number8
DOIs
Publication statusPublished - Aug 2015

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Keywords

  • Alternative pre-mRNA splicing
  • Transcriptomics

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