Low-cost high sensitivity pulsed endomicroscopy to visualize tricolor optical signatures

Nikola Krstajić, Bethany Mills, Ian Murray, Adam Marshall, Dominic Norberg, Thomas H. Craven, Philip Emanuel, Tushar R. Choudhary, Gareth O. S. Williams, Emma Scholefield, Ahsan R. Akram, Andrew Davie, Nik Hirani, Annya Bruce, Anne Moore, Mark Bradley, Kevin Dhaliwal

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    11 Citations (Scopus)
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    Abstract

    A highly sensitive, modular three-color fluorescence endomicroscopy imaging platform spanning the visible to near-infrared (NIR) range is demonstrated. Light-emitting diodes (LEDs) were sequentially pulsed along with the camera acquisition to provide up to 20 frames per second (fps) three-color imaging performance or 60 fps single color imaging. The system was characterized for bacterial and cellular molecular imaging in ex vivo human lung tissue and for bacterial and indocyanine green imaging in ex vivo perfused sheep lungs. A practical method to reduce background tissue autofluorescence is also proposed. The platform was clinically translated into six patients with pulmonary disease to delineate healthy, cancerous, and fibrotic tissue autofluorescent structures. The instrument is the most broadband clinical endomicroscopy system developed to date (covering visible to the NIR, 500 to 900 nm) and demonstrates significant potential for future clinical utility due to its low cost and modular capability to suit a wide variety of molecular imaging applications.

    Original languageEnglish
    Article number076005
    Number of pages12
    JournalJournal of Biomedical Optics
    Volume23
    Issue number7
    DOIs
    Publication statusPublished - 10 Jul 2018

    Keywords

    • endomicroscopy
    • fluorescence
    • lung
    • microendoscopy
    • molecular imaging
    • respiratory medicine

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