@article{04a2e5c463ca40549d21dc74bdd22f0e,
title = "LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages",
abstract = "Mutations in the leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson's disease, chronic inflammation and mycobacterial infections. Although there is evidence supporting the idea that LRRK2 has an immune function, the cellular function of this kinase is still largely unknown. By using genetic, pharmacological and proteomics approaches, we show that LRRK2 kinase activity negatively regulates phagosome maturation via the recruitment of the Class III phosphatidylinositol-3 kinase complex and Rubicon to the phagosome in macrophages. Moreover, inhibition of LRRK2 kinase activity in mouse and human macrophages enhanced Mycobacterium tuberculosis phagosome maturation and mycobacterial control independently of autophagy. In vivo, LRRK2 deficiency in mice resulted in a significant decrease in M. tuberculosis burdens early during the infection. Collectively, our findings provide a molecular mechanism explaining genetic evidence linking LRRK2 to mycobacterial diseases and establish an LRRK2-dependent cellular pathway that controls M. tuberculosis replication by regulating phagosome maturation.",
keywords = "LRRK2, Parkinson's disease, Rubicon, phagosome, tuberculosis",
author = "Anetta H{\"a}rtlova and Susanne Herbst and Julien Peltier and Angela Rodgers and Orsolya Bilkei-Gorzo and Antony Fearns and Dill, {Brian D.} and Heyne Lee and Rowan Flynn and Cowley, {Sally A.} and Paul Davies and Lewis, {Patrick A.} and Ganley, {Ian G.} and Jennifer Martinez and Alessi, {Dario R.} and Reith, {Alastair D.} and Matthias Trost and Gutierrez, {Maximiliano G.}",
note = "This work was funded by Medical Research Council UK (MR/N026004/1 and MR/L010933/1 to PAL, MC_UU_12016/5 to MT and MC_UP_1202/11 to MGG); the pharmaceutical companies supporting the Division of Signal Transduction Therapy Unit (Boehringer Ingelheim, GSK and Merck KGaA, to MT); the Michael J. Fox Foundation (to MGG and PAL), Parkinson{\textquoteright}s UK Fellowship F1002 to PAL and the Francis Crick Institute (to MGG), which receives its core funding from Cancer Research UK (FC001092), the Medical Research Council UK (FC001092) and the Wellcome Trust (FC001092); the Oxford Martin School (LC0910-004) and the Wellcome Trust (WTISSF121302) to SAC; the Innovative Medicines Initiative Joint Undertaking (IMI JU, 115439), the European Union{\textquoteright}s Seventh Framework Programme, EFPIA companies{\textquoteright} in kind contribution to SAC",
year = "2018",
month = jun,
day = "15",
doi = "10.15252/embj.201798694",
language = "English",
volume = "37",
pages = "1--17",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "EMBO Press",
number = "12",
}