MacroH2A allows ATP-dependent chromatin remodeling by SWI/SNF and ACF complexes but specifically reduces recruitment of SWI/SNF

Evelyn Y. Chang, Helder Ferreira, Joanna Somers, Dmitri A. Nusinow, Tom Owen-Hughes, Geeta J. Narlikar

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    37 Citations (Scopus)

    Abstract

    The variant histone macroH2A helps maintain X inactivation and gene silencing. Previous work implied that nucleosomes containing macroH2A cannot be remodeled by ISWI and SWI/SNF chromatin remodeling enzymes. Using approaches that prevent misassembly of macroH2A nucleosomes, we find that macroH2A nucleosomes are excellent substrates for both enzyme families. Interestingly, SWI/SNF, which is involved in gene activation, preferentially binds H2A nucleosomes over macroH2A nucleosomes, but ACF, an ISWI complex implicated in gene repression, shows no preference. Thus, macroH2A may help regulate the balance between activating and repressive remodeling complexes.

    Original languageEnglish
    Pages (from-to)13726-13732
    Number of pages7
    JournalBiochemistry
    Volume47
    Issue number51
    DOIs
    Publication statusPublished - 23 Dec 2008

    Keywords

    • HISTONE VARIANT MACROH2A
    • GENE-EXPRESSION
    • X-CHROMOSOME
    • IN-VIVO
    • INACTIVATION
    • NUCLEOSOMES
    • MECHANISM
    • FAMILY
    • DRIVEN
    • SNF2H

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