TY - JOUR
T1 - Magnetic nanoparticles as intraocular drug delivery system to target Retinal Pigmented Epithelium (RPE)
AU - Giannaccini, Martina
AU - Giannini, Marianna
AU - Calatayud, M. Pilar
AU - Goya, Gerardo F.
AU - Cuschieri, Alfred
AU - Dente, Luciana
AU - Raffa, Vittoria
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/1/22
Y1 - 2014/1/22
N2 - One of the most challenging efforts in drug delivery is the targeting of the eye. The eye structure and barriers render this organ poorly permeable to drugs. Quite recently the entrance of nanoscience in ocular drug delivery has improved the penetration and half-life of drugs, especially in the anterior eye chamber, while targeting the posterior chamber is still an open issue. The retina and the retinal pigment epithelium/choroid tissues, located in the posterior eye chamber, are responsible for the majority of blindness both in childhood and adulthood. In the present study, we used magnetic nanoparticles (MNPs) as a nanotool for ocular drug delivery that is capable of specific localization in the retinal pigmented epithelium (RPE) layer. We demonstrate that, following intraocular injection in Xenopus embryos, MNPs localize specifically in RPE where they are retained for several days. The specificity of the localization did not depend on particle size and surface properties of the MNPs used in this work. Moreover, through similar experiments in zebrafish, we demonstrated that the targeting of RPE by the nanoparticles is not specific for the Xenopus species.
AB - One of the most challenging efforts in drug delivery is the targeting of the eye. The eye structure and barriers render this organ poorly permeable to drugs. Quite recently the entrance of nanoscience in ocular drug delivery has improved the penetration and half-life of drugs, especially in the anterior eye chamber, while targeting the posterior chamber is still an open issue. The retina and the retinal pigment epithelium/choroid tissues, located in the posterior eye chamber, are responsible for the majority of blindness both in childhood and adulthood. In the present study, we used magnetic nanoparticles (MNPs) as a nanotool for ocular drug delivery that is capable of specific localization in the retinal pigmented epithelium (RPE) layer. We demonstrate that, following intraocular injection in Xenopus embryos, MNPs localize specifically in RPE where they are retained for several days. The specificity of the localization did not depend on particle size and surface properties of the MNPs used in this work. Moreover, through similar experiments in zebrafish, we demonstrated that the targeting of RPE by the nanoparticles is not specific for the Xenopus species.
KW - Intraocular delivery
KW - Magnetic Nanoparticle (MNP)
KW - Retinal Pigmented Epithelium (RPE)
KW - Xenopus laevis
UR - http://www.scopus.com/inward/record.url?scp=84896738399&partnerID=8YFLogxK
U2 - 10.3390/ijms15011590
DO - 10.3390/ijms15011590
M3 - Article
C2 - 24451140
AN - SCOPUS:84896738399
VL - 15
SP - 1590
EP - 1605
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 1
ER -