Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA

Anne Ramat, Matthew Hannaford, Jens Januschke (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)
    255 Downloads (Pure)

    Abstract

    How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis. We then used GFP-specific nanobodies fused to localization domains to alter the subcellular distribution of the GFP-tagged mRNA or protein. Altering the localization of the mRNA resulted in mislocalization of the protein and vice versa. Protein localization defects caused by mislocalization of the cognate mRNA were rescued by introducing untagged mRNA coding for mutant non-localizable protein. Therefore by combining the MS2 system and subcellular nanobody expression we uncovered that maintenance of Mira asymmetric localization requires interaction with the cognate mRNA.
    Original languageEnglish
    Pages (from-to)2101-2015
    Number of pages16
    JournalCurrent Biology
    Volume27
    Issue number14
    Early online date6 Jul 2017
    DOIs
    Publication statusPublished - 6 Jul 2017

    Keywords

    • Drosophila
    • Asymmetric cell division
    • mRNA localization
    • Nanobody
    • Neuroblasts
    • Polarity

    Fingerprint

    Dive into the research topics of 'Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA'. Together they form a unique fingerprint.

    Cite this