Manganese enhances prion protein survival in model soils and increases prion infectivity to cells.

P Davies; David  Brown

doi:10.1371/journal.pone.0007518

Manganese enhances prion protein survival in model soils and increases prion infectivity to cells.

P Davies
, David Brown (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

6 Downloads (Pure)

Abstract

Prion diseases are considered to be transmissible. The existence of sporadic forms of prion diseases such as scrapie implies an environmental source for the infectious agent. This would suggest that under certain conditions the prion protein, the accepted agent of transmission, can survive in the environment. We have developed a novel technique to extract the prion protein from soil matrices. Previous studies have suggested that environmental manganese is a possible risk factor for prion diseases. We have shown that exposure to manganese is a soil matrix causes a dramatic increase in prion protein survival (∼10 fold) over a two year period. We have also shown that manganese increases infectivity of mouse passaged scrapie to culture cells by 2 logs. These results clearly verify that manganese is a risk factor for both the survival of the infectious agent in the environment and its transmissibility.

Original language	English
Article number	e7518
Journal	PLoS ONE
Volume	4
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0007518
Publication status	Published - 21 Oct 2009

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10.1371/journal.pone.0007518Licence: CC BY

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abstract = "Prion diseases are considered to be transmissible. The existence of sporadic forms of prion diseases such as scrapie implies an environmental source for the infectious agent. This would suggest that under certain conditions the prion protein, the accepted agent of transmission, can survive in the environment. We have developed a novel technique to extract the prion protein from soil matrices. Previous studies have suggested that environmental manganese is a possible risk factor for prion diseases. We have shown that exposure to manganese is a soil matrix causes a dramatic increase in prion protein survival (∼10 fold) over a two year period. We have also shown that manganese increases infectivity of mouse passaged scrapie to culture cells by 2 logs. These results clearly verify that manganese is a risk factor for both the survival of the infectious agent in the environment and its transmissibility.",

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Manganese enhances prion protein survival in model soils and increases prion infectivity to cells.

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