MAP kinase inactivation is required only for G2-M phase transition in early embryogenesis cell cycles of the starfishes Marthasterias glacialis and Astropecten aranciacus

Daniel Fisher, Ariane Abrieu, Marie-Noelle Simon, Stephen Keyse, Valerie Vergé, Marcel Dorée, Andre Picard

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Downregulation of MAP kinase is a universal consequence of fertilization in the animal kingdom. Here we show that oocytes of the starfishes Astropecten aranciacus and Marthasterias glacialis complete meiotic maturation and form a pronucleus when treated with 1-methyladenine and then complete DNA replication and arrest at G2 if not fertilized. Release of G2 by fertilization or a variety of parthenogenetic treatments is associated with inactivation of MAP kinase. Prevention of MAP kinase inactivation by microinjection of Ste11-DeltaN, a constitutively active budding yeast MAP kinase kinase kinase, arrests fertilized eggs at G2 in either the first or the second mitotic cell cycle, in a dose-dependent manner. G1 arrest is never observed. Conversely, inactivation of MAP kinase by microinjection of the MAP kinase-specific phosphatase Pyst-1 releases mature starfish oocytes from G2 arrest. The role of MAP kinase in arresting cell cycle at various stages in oocytes of different animal species is discussed.
    Original languageEnglish
    Pages (from-to)1-13
    Number of pages13
    JournalDevelopmental Biology
    Volume202
    Issue number1
    DOIs
    Publication statusPublished - 1 Oct 1998

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