Mapping and expression of genes associated with raspberry fruit ripening and softening

Craig G. Simpson (Lead / Corresponding author), Danny W. Cullen, Christine A. Hackett, Kay Smith, Paul D. Hallett, Jim McNicol, Mary Woodhead, Julie Graham

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

KEY MESSAGE: QTL mapping identifies a range of underlying and unrelated genes with apparent roles in raspberry fruit ripening and softening that show characteristic developing fruit expression profiles. Fruit softening is an important agronomical trait that involves a complex interaction of plant cell processes. We have used both qualitative and quantitative scoring of fruit firmness, length, mass, and resistance to applied force to identify QTL in a raspberry mapping population. QTLs were located primarily on linkage group (LG) 3 with other significant loci on LG 1 and LG 5 and showed mostly additive effects between the two parents. The expression of key genes that underlie these QTLs with roles in cell-wall solubility, water uptake, polyamine synthesis, transcription, and cell respiration was tested across five stages of fruit development, from immature green to red ripe fruit, using real-time RT-qPCR. Gene expression patterns showed variable expression patterns across fruit development with a highly significant positive and negative correlation between genes, supporting precise regulation of expression of different cell processes throughout raspberry fruit development. Variable timing in expression was also found in some genes at different fruit development stages between soft and firm cultivars. Multiple processes have a role to play in fruit softening and this will require development of multiple marker combinations to genes that characterise raspberry fruit softening.

Original languageEnglish
Pages (from-to)557-572
Number of pages16
JournalTheoretical and Applied Genetics
Volume130
Issue number3
Early online date9 Dec 2016
DOIs
Publication statusPublished - Mar 2017

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Chromosome Mapping
raspberries
chromosome mapping
Fruit
ripening
Gene Expression
gene expression
fruits
fruiting
quantitative trait loci
linkage groups
genes
Genes
polyamines
Rubus
cell respiration
additive effect
water uptake
firmness
Cell Respiration

Cite this

Simpson, C. G., Cullen, D. W., Hackett, C. A., Smith, K., Hallett, P. D., McNicol, J., ... Graham, J. (2017). Mapping and expression of genes associated with raspberry fruit ripening and softening. Theoretical and Applied Genetics, 130(3), 557-572. https://doi.org/10.1007/s00122-016-2835-7
Simpson, Craig G. ; Cullen, Danny W. ; Hackett, Christine A. ; Smith, Kay ; Hallett, Paul D. ; McNicol, Jim ; Woodhead, Mary ; Graham, Julie. / Mapping and expression of genes associated with raspberry fruit ripening and softening. In: Theoretical and Applied Genetics. 2017 ; Vol. 130, No. 3. pp. 557-572.
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Simpson, CG, Cullen, DW, Hackett, CA, Smith, K, Hallett, PD, McNicol, J, Woodhead, M & Graham, J 2017, 'Mapping and expression of genes associated with raspberry fruit ripening and softening', Theoretical and Applied Genetics, vol. 130, no. 3, pp. 557-572. https://doi.org/10.1007/s00122-016-2835-7

Mapping and expression of genes associated with raspberry fruit ripening and softening. / Simpson, Craig G. (Lead / Corresponding author); Cullen, Danny W.; Hackett, Christine A.; Smith, Kay; Hallett, Paul D.; McNicol, Jim; Woodhead, Mary; Graham, Julie.

In: Theoretical and Applied Genetics, Vol. 130, No. 3, 03.2017, p. 557-572.

Research output: Contribution to journalArticle

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N1 - We acknowledge funding for this research from the Scottish Government Rural and Environment Science and Analytical Services division (RESAS) and Horticultural LINK programme (Project No.: HL0195). We thank UK Raspberry Breeding Consortium members HDC, T. Thomson Blairgowrie Ltd, MRS, Delta-T, M&S plc and ReDeva for their valuable support.

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N2 - KEY MESSAGE: QTL mapping identifies a range of underlying and unrelated genes with apparent roles in raspberry fruit ripening and softening that show characteristic developing fruit expression profiles. Fruit softening is an important agronomical trait that involves a complex interaction of plant cell processes. We have used both qualitative and quantitative scoring of fruit firmness, length, mass, and resistance to applied force to identify QTL in a raspberry mapping population. QTLs were located primarily on linkage group (LG) 3 with other significant loci on LG 1 and LG 5 and showed mostly additive effects between the two parents. The expression of key genes that underlie these QTLs with roles in cell-wall solubility, water uptake, polyamine synthesis, transcription, and cell respiration was tested across five stages of fruit development, from immature green to red ripe fruit, using real-time RT-qPCR. Gene expression patterns showed variable expression patterns across fruit development with a highly significant positive and negative correlation between genes, supporting precise regulation of expression of different cell processes throughout raspberry fruit development. Variable timing in expression was also found in some genes at different fruit development stages between soft and firm cultivars. Multiple processes have a role to play in fruit softening and this will require development of multiple marker combinations to genes that characterise raspberry fruit softening.

AB - KEY MESSAGE: QTL mapping identifies a range of underlying and unrelated genes with apparent roles in raspberry fruit ripening and softening that show characteristic developing fruit expression profiles. Fruit softening is an important agronomical trait that involves a complex interaction of plant cell processes. We have used both qualitative and quantitative scoring of fruit firmness, length, mass, and resistance to applied force to identify QTL in a raspberry mapping population. QTLs were located primarily on linkage group (LG) 3 with other significant loci on LG 1 and LG 5 and showed mostly additive effects between the two parents. The expression of key genes that underlie these QTLs with roles in cell-wall solubility, water uptake, polyamine synthesis, transcription, and cell respiration was tested across five stages of fruit development, from immature green to red ripe fruit, using real-time RT-qPCR. Gene expression patterns showed variable expression patterns across fruit development with a highly significant positive and negative correlation between genes, supporting precise regulation of expression of different cell processes throughout raspberry fruit development. Variable timing in expression was also found in some genes at different fruit development stages between soft and firm cultivars. Multiple processes have a role to play in fruit softening and this will require development of multiple marker combinations to genes that characterise raspberry fruit softening.

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