Abstract
Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.
| Original language | English |
|---|---|
| Pages (from-to) | 652-660 |
| Number of pages | 9 |
| Journal | Theoretical and Applied Genetics |
| Volume | 101 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Sep 2000 |
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Keywords
- Barley
- Genetic similarity (GS)
- Linkage map
- Microsatellites
- Polymorphism information content (PIC)
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Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars. / Pillen, K. (Lead / Corresponding author); Binder, A.; Kreuzkam, B.; Ramsay, L.; Waugh, R.; Förster, J.; Léon, J.
In: Theoretical and Applied Genetics, Vol. 101, No. 4, 09.2000, p. 652-660.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars
AU - Pillen, K.
AU - Binder, A.
AU - Kreuzkam, B.
AU - Ramsay, L.
AU - Waugh, R.
AU - Förster, J.
AU - Léon, J.
PY - 2000/9
Y1 - 2000/9
N2 - Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.
AB - Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.
KW - Barley
KW - Genetic similarity (GS)
KW - Linkage map
KW - Microsatellites
KW - Polymorphism information content (PIC)
UR - http://www.scopus.com/inward/record.url?scp=0033800707&partnerID=8YFLogxK
U2 - 10.1007/s001220051527
DO - 10.1007/s001220051527
M3 - Article
AN - SCOPUS:0033800707
VL - 101
SP - 652
EP - 660
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
SN - 0040-5752
IS - 4
ER -