Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars

K. Pillen (Lead / Corresponding author), A. Binder, B. Kreuzkam, L. Ramsay, R. Waugh, J. Förster, J. Léon

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.

Original languageEnglish
Pages (from-to)652-660
Number of pages9
JournalTheoretical and Applied Genetics
Volume101
Issue number4
DOIs
Publication statusPublished - Sep 2000

Fingerprint

Hordeum
Microsatellite Repeats
barley
microsatellite repeats
cultivars
winter barley
genetic polymorphism
genome
spring barley
Genome
research projects
polymorphism
Nucleic Acid Databases
screening
chromosomes
Triticum
nucleotide sequences
wheat
winter
Chromosomes

Keywords

  • Barley
  • Genetic similarity (GS)
  • Linkage map
  • Microsatellites
  • Polymorphism information content (PIC)

Cite this

Pillen, K. ; Binder, A. ; Kreuzkam, B. ; Ramsay, L. ; Waugh, R. ; Förster, J. ; Léon, J. / Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars. In: Theoretical and Applied Genetics. 2000 ; Vol. 101, No. 4. pp. 652-660.
@article{4cab17dc0ac140e38fee644b89eb683c,
title = "Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars",
abstract = "Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.",
keywords = "Barley, Genetic similarity (GS), Linkage map, Microsatellites, Polymorphism information content (PIC)",
author = "K. Pillen and A. Binder and B. Kreuzkam and L. Ramsay and R. Waugh and J. F{\"o}rster and J. L{\'e}on",
year = "2000",
month = "9",
doi = "10.1007/s001220051527",
language = "English",
volume = "101",
pages = "652--660",
journal = "Theoretical and Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "4",

}

Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars. / Pillen, K. (Lead / Corresponding author); Binder, A.; Kreuzkam, B.; Ramsay, L.; Waugh, R.; Förster, J.; Léon, J.

In: Theoretical and Applied Genetics, Vol. 101, No. 4, 09.2000, p. 652-660.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars

AU - Pillen, K.

AU - Binder, A.

AU - Kreuzkam, B.

AU - Ramsay, L.

AU - Waugh, R.

AU - Förster, J.

AU - Léon, J.

PY - 2000/9

Y1 - 2000/9

N2 - Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.

AB - Abstract By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL micro-satellites were mapped either to the interspecific barley map LerchexBGRC41936 (Lx41), the IgrixFranka map (IxF, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic Similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.

KW - Barley

KW - Genetic similarity (GS)

KW - Linkage map

KW - Microsatellites

KW - Polymorphism information content (PIC)

UR - http://www.scopus.com/inward/record.url?scp=0033800707&partnerID=8YFLogxK

U2 - 10.1007/s001220051527

DO - 10.1007/s001220051527

M3 - Article

AN - SCOPUS:0033800707

VL - 101

SP - 652

EP - 660

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

IS - 4

ER -